The Effects of Exercise Preconditioning on Cerebral Blood Flow Change and Endothelin-1 Expression after Cerebral Ischemia in Rats
The Effects of Exercise Preconditioning on Cerebral Blood Flow Change and Endothelin-1 Expression after Cerebral Ischemia in Rats
复制标题
运动预处理对大鼠脑缺血后脑血流变化及内皮素1表达的影响
DOI：
10.1016/j.jstrokecerebrovasdis.2014.01.016
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发表时间：
2014-07-01
影响因子：
2.5
通讯作者：
Jia, Jie
中科院分区：
文献类型：
--
作者：
Zhang, Qi;Zhang, Li;Jia, Jie
脑卒中是一种发病率、致残率和死亡率都很高的急性脑血管病。缺血前跑台训练已被证明能有效改善脑缺血后的行为和神经病理指标。然而，缺血前跑台训练对缺血性损伤的确切保护机制尚未阐明。本研究探讨缺血前跑台训练是否能通过调节脑血流量（CBF）和内皮素1（ET-1）对脑缺血损伤起到保护作用。我们分析了CBF的激光散斑成像和ET-1表达的酶联免疫吸附试验使用缺血大鼠模型与缺血前跑台训练。总体而言，运动前组ET-1表达明显降低，CBF明显升高。值得注意的是，与大脑中动脉闭塞后的时间水平相比，跑步前组再灌注24小时的ET-1表达增加。这些变化之后，神经功能缺损和脑梗死体积的显着变化。本研究提示，运动预适应通过改善脑血流量和调节ET-1的表达对脑缺血损伤具有保护作用，这可能是运动预缺血对脑损伤的神经保护机制的一个新组成部分。
Stroke is an acute cerebrovascular disease with high incidence, morbidity, and mortality. Preischemic treadmill training has been shown to be effective in improving behavioral and neuropathologic indices after cerebral ischemia. However, the exact neuroprotective mechanism of preischemic treadmill training against ischemic injury has not been elucidated clearly. The present study investigated whether preischemic treadmill training could protect the brain from ischemic injury via regulating cerebral blood flow (CBF) and endothelin 1 (ET-1). We analyzed the CBF by laser speckle imaging and ET-1 expression by an enzyme-linked immunosorbent assay using an ischemic rat model with preischemic treadmill training. Generally speaking, ET-1 expression decreased and CBF increased significantly in the pretreadmill group. It is worth noting that ET-1 expression is increased at 24 hours of reperfusion in the pretreadmill group compared with the level of the time after middle cerebral artery occlusion. These changes were followed by significant changes in neurologic deficits and cerebral infarct volume. This study indicated that preconditioning exercise protected brain from ischemic injury through the improvement of CBF and regulation of ET-1 expression, which may be a novel component of the neuroprotective mechanism of preischemic treadmill training against brain injury.