A lamin B receptor in the nuclear envelope.
A lamin B receptor in the nuclear envelope.
复制标题
核膜中的核纤层蛋白 B 受体。
DOI：
10.1073/pnas.85.22.8531
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发表时间：
1988
影响因子：
11.1
通讯作者：
Georgatos,SD
中科院分区：
文献类型：
--
作者：
Worman,HJ;Yuan,J;Blobel,G;Georgatos,SD
使用溶液结合试验，我们表明，纯化的125 I-标记的核纤层蛋白B结合在一个饱和和特定的方式，核纤层蛋白耗尽禽红细胞核膜的Kd约为0.2 μ M。这种结合是显着大于125 I-标记的核纤层蛋白A的结合，并竞争性抑制未标记的配体。我们证明，一个58 kDa的整合膜蛋白（p58）是核纤层蛋白B受体凭借其丰富的核膜和协会与125 I-标记的核纤层蛋白B在配体印迹分析。针对p58的特异性抗体识别分离的细胞核中的一种蛋白质，并部分阻断125 I标记的核纤层蛋白B与核纤层蛋白耗尽的核膜的结合。细胞分级分离和间接免疫荧光显微镜显示，p58位于细胞核的周边。该蛋白质可作为核纤层的膜附着位点，作为核纤层蛋白B的特异性受体。
Using a solution binding assay, we show that purified 125I-labeled lamin B binds in a saturable and specific fashion to lamin-depleted avian erythrocyte nuclear membranes with a Kd of approximately 0.2 microM. This binding is significantly greater than the binding of 125I-labeled lamin A and is competitively inhibited by unlabeled ligand. We demonstrate that a 58-kDa integral membrane protein (p58) is a lamin B receptor by virtue of its abundance in the nuclear envelope and association with 125I-labeled lamin B in ligand blotting assays. Specific antibodies raised against p58 recognize one protein in isolated nuclei and partially block 125I-labeled lamin B binding to lamin-depleted nuclear membranes. Cell fractionation and indirect immunofluorescence microscopy show that p58 is located in the periphery of the nucleus. This protein may serve as a membrane attachment site for the nuclear lamina by acting as a specific receptor for lamin B.