Identification of Cupressaceae species from airborne pollen grains using chloroplastic markers: implications for reproductive interference evaluation in a remnant natural population of Chamaecyparis pisifera (Sieb. et Zucc.) Endl
Identification of Cupressaceae species from airborne pollen grains using chloroplastic markers: implications for reproductive interference evaluation in a remnant natural population of Chamaecyparis pisifera (Sieb. et Zucc.) Endl
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使用叶绿体标记从空气传播的花粉粒中鉴定柏科物种：对剩余自然种群 Chamaecyparis pisifera (Sieb. et Zucc.) 生殖干扰评估的影响 Endl
DOI：
10.1080/13416979.2017.1368155
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发表时间：
2017
影响因子：
1.5
通讯作者：
Momi Tsuruta & Yuzuru Mukai
中科院分区：
文献类型：
--
作者：
Akihiro Ikeru;Momi Tsuruta & Yuzuru Mukai
许多野生种群的Chamaanoparis pisifera（柏科）最近支离破碎，周围的针叶林。为了区分散粉到C.根据叶绿体DNA内含子L（UAA）区域的差异，开发了两个遗传标记（Chaps-trnL 01和Chaps-trnL 02）。该地区在C。pisifer含有ClaI限制性内切酶的位点，可以区分C. pisifera从其他柏科物种的酶消化后的产物，导致切割扩增多态性序列（CAPS）标记的发展。标记的可用性通过单花粉基因分型来确定。结果表明，在上述3种花粉中，扩增成功率分别为75.0%、58.3%和100%。pisifera、Chamaaparis obtusa和柳杉（Cryptomeria japonica）。然后，将标记和基因分型方法应用于从一个残留的天然林中采集的气传花粉。豌豆。90%以上的花粉被鉴定为C.钝角。因此，我们认为，大量的异种花粉传播存在于开花期。豌豆。这些结果表明，残余C。pisiferapustomy可能会受到生殖干扰，由于较低的机会，与同种花粉授粉。
Many wild populations ofChamaecyparis pisifera(Cupressaceae) are recently fragmented and surrounded by coniferous plantations. To distinguish the species composition of pollens that are dispersed to female flowers ofC. pisifera, genetic markers (Chaps-trnL01 and Chaps-trnL02) were developed based on the differences intrnL (UAA) intron region of chloroplast DNA. This region inC. pisiferacontains the site of theClaI restriction enzyme and allows the distinction ofC. pisiferafrom other Cupressaceae species after digestion of the products with the enzyme resulting in the development of cleaved amplified polymorphic sequence (CAPS) markers. The availability of markers was determined by single-pollen genotyping. Amplification success rates of 75.0, 58.3, and 100% were obtained for the pollens ofC. pisifera, Chamaecyparis obtusa, andCryptomeria japonica, respectively. Then, the markers and genotyping methods were applied to airborne pollen grains sampled from a remnant natural stand ofC. pisifera. Over 90% of the pollen grains were identified as belonging toC. obtusa. Thus, we concluded that abundant heterospecific pollen dispersal was present during the flowering periods ofC. pisifera. These results suggest that the remnantC. pisiferapopulation might suffer reproductive interference due to the lower chances to be pollinated with conspecific pollens.