EFFECT OF COLCHICINE ON THE UPTAKE OF PROLACTIN AND INSULIN INTO GOLGI FRACTIONS OF RAT-LIVER
EFFECT OF COLCHICINE ON THE UPTAKE OF PROLACTIN AND INSULIN INTO GOLGI FRACTIONS OF RAT-LIVER
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DOI：
10.1083/jcb.93.3.560
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发表时间：
1982-01-01
影响因子：
7.8
通讯作者：
BERGERON, JJM
中科院分区：
文献类型：
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作者：
POSNER, BI;VERMA, AK;BERGERON, JJM
~(125)I标记的催乳素通过受体依赖的过程被摄取，并以完整的形式集中在雌性大鼠肝脏的高尔基体中。秋水仙素对这一摄取过程中高尔基体元素的影响进行了检验。秋水仙碱[25微克(10毫克)/100克体重]，ip。成年雌性大鼠，分别于注射后1、2、3h制备肝脏高尔基体组分。秋水仙素各组分的酶恢复和形态观察结果表明，~(125)I-绵羊催乳素(~(125)I-oPRL)对高尔基体轻组分和中间组分的摄取有明显的抑制作用，而对高尔基体重组分和质膜组分的摄取没有明显的抑制作用。间歇性地，从注射后2-45分钟开始，从高尔基体中提取~(125)I-oPRL，并通过与受体的重新结合来判断其基本完整。胆碱在0.25-25微克/100g体重范围内有抑制作用，长春新碱也能抑制~(125)I-oPRL在高尔基体光和中间体的摄取，而鲁米诺水仙碱则无抑制作用。秋水仙碱早期(1h)和晚期(3h)对~(125)I-胰岛素摄取的幅度和模式的影响较小。秋水仙素处理后，高尔基体组分中的乳素受体水平没有发生显著变化。秋水仙素处理抑制了催乳素向高尔基体泡囊分子的转移。对胰岛素摄取的影响要小得多，这表明这两种激素在内化过程中的处理方式可能有所不同。
125I-labeled prolactin is taken up by a receptor-dependent process and concentrated in an intact form in Golgi elements from female rat liver. The effect of colchicine on this uptake process into Golgi elements is examined. Colchicine [25 .mu.mol (10 mg)/100 gm body wt] was injected i.p. in adult female rats, and hepatic Golgi fractions were prepared at 1, 2 and 3 h postinjection. The enzyme recoveries and morphological appearance of fractions from colchicine there was a marked (> 60%) inhibition of uptake of 125I-ovine prolactin (125I-oPRL) into Golgi light and intermediate fractions but no inhibition of uptake into Golgi heavy and plasmalemma elements. Intermittently, from 2-45 min postinjection, 125I-oPRL was extracted from Golgi elements and found to be largely intact as judged by rebinding to receptors. The inhibitory effect of cholchicine was seen at doses ranging from 0.25-25 .mu.mol/100 g body wt. Vincristine also inhibited 125I-oPRL uptake into the Golgi light and intermediate fractions but lumicolchicine had no inhibitory effect. There was a smaller effect of colchicine at early (1 h) and later (3 h) times on the extent and pattern of 125I-insulin uptake. Colchicine treatment did not produce a significant change in lactogen receptor levels in the Golgi fractions. Colchicine treatment inhibited the transfer of prolactin into Golgi vesicular elements. The much smaller effect on insulin uptake suggests that there may be differences in the manner in which the 2 hormones are handled in the course of internalization.