High-Resolution Ultrasound Imaging A Novel Technique for the Noninvasive in Vivo Analysis of Endometriotic Lesion and Cyst Formation in Small Animal Models
High-Resolution Ultrasound Imaging A Novel Technique for the Noninvasive in Vivo Analysis of Endometriotic Lesion and Cyst Formation in Small Animal Models
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DOI：
10.2353/ajpath.2010.090617
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发表时间：
2010-02-01
影响因子：
6
通讯作者：
Menger, Michael D.
中科院分区：
文献类型：
--
作者：
Laschke, Matthias W.;Koerbel, Christina;Menger, Michael D.
子宫内膜异位症是一种高度常见的妇科疾病，严重影响患者的生活质量。为了分析疾病的发病机制，并为有效的治疗寻找新的分子靶点，小动物模型是一个重要的方法。在此，我们报告了首次使用高分辨率超声成像对小鼠腹膜内子宫内膜异位症病变进行活体分析。这种非侵入性技术可以重复定量分析子宫内膜异位症病变的生长、囊变和粘连形成，观察者内和观察者间的变异性很小。此外，它使人们能够很容易地区分子宫内膜囊肿和间质。相应地，子宫内膜囊和间质的体积测量表明，子宫内膜异位病变的最初建立与细胞增殖增强有关，随后是子宫内膜腺分泌活动增加的阶段。超声分析的结果与卡尺和组织学测量的病变体积有很好的相关性。重要的是，超声成像可以重复和非侵入性地进行，最能反映体内的情况。这项技术可以进一步证明，在特定的雌激素受体去稳定剂治疗后，该技术可以成功地监测子宫内膜异位症病变生长的显着抑制。因此，高分辨率超声成像是未来临床前小动物研究的重要工具，这些研究涉及子宫内膜异位症的病理生理学和新治疗策略的开发。(Am J Pathol2010,176：585-593；DOI：10.2353/ajpath.2010.090617)
Endometriosis, the presence of endometrial tissue at ectopic sites, is a highly prevalent gynecological disease severely affecting a patient's quality of life. To analyze the mechanisms involved in the disease and to identify new molecular targets for effective therapies, small animal models are an important approach. Herein, we report the first use of high-resolution ultrasound imaging for the in vivo analysis of intraperitoneal endometriotic lesions in mice. This noninvasive technology allows for the repetitive quantitative analysis of growth, cyst development, and adhesion formation of endometriotic lesions with a low intra- and interobserver variability. Moreover, it enables one to easily differentiate between endometrial cysts and stroma. Accordingly, volume measurements of both endometrial cysts and stroma indicated that the initial establishment of endometriotic lesions is associated with enhanced cellular proliferation, followed by a phase of increased secretory activity of endometrial glands. Results of ultrasound analysis correlated well with measurements of lesion volumes by caliper and histology. importantly, ultrasound imaging could be performed repetitively and noninvasively and reflected best the in vivo situation. The technique could further he demonstrated to successfully monitor the significant inhibition of growth of endometriotic lesions after specific estrogen receptor destabilizator treatment. Thus, high-resolution ultrasound imaging represents an important tool for future preclinical small animal studies, which address the pathophysiology of endometriosis and the development of new treatment strategies. (Am J Pathol 2010, 176:585-593; DOI: 10.2353/ajpath.2010.090617)