GLUTAMATE RECEPTOR-LIKE genes mediate leaf-to-leaf wound signalling
GLUTAMATE RECEPTOR-LIKE genes mediate leaf-to-leaf wound signalling
复制标题
DOI：
10.1038/nature12478
复制
发表时间：
2013-08-22
期刊：
影响因子：
64.8
通讯作者：
Farmer, Edward E.
中科院分区：
文献类型：
--
作者：
Mousavi, Seyed A. R.;Chauvin, Adeline;Farmer, Edward E.
受伤的叶片通过一个鲜为人知的长距离信号传递过程相互传递它们的损伤状态。这刺激了远端茉莉酸盐的产生，这是防御反应的有效调节剂。我们利用非侵入性电极绘制了拟南芥叶片8损伤后的表面电位变化，发现未损伤叶片的膜去极化与茉莉酸盐信号域相关。此外，电流注射引起茉莉异亮氨酸积累，导致编码关键茉莉酸信号调节因子的rna转录组富集。在筛选的34个膜蛋白突变系中，几个3枝谷氨酸受体样基因（GLRs 3.2、3.3和3.6）的突变减弱了伤口诱导的表面电位变化。在glr3.3 glr3.6双突变体中，伤口远端叶片中茉莉酸反应基因的表达减少。该研究为研究植物长距离创伤信号传导机制提供了遗传学基础，并表明动物突触活性相关基因在器官-器官创伤信号传导中发挥着重要作用。
Wounded leaves communicate their damage status to one another through a poorly understood process of long-distance signalling. This stimulates the distal production of jasmonates, potent regulators of defence responses. Using non-invasive electrodes we mapped surface potential changes in Arabidopsis thaliana after wounding leaf eight and found that membrane depolarizations correlated with jasmonate signalling domains in undamaged leaves. Furthermore, current injection elicited jasmonoyl-isoleucine accumulation, resulting in a transcriptome enriched in RNAs encoding key jasmonate signalling regulators. From among 34 screened membrane protein mutant lines, mutations in several clade 3 GLUTAMATE RECEPTOR-LIKE genes (GLRs 3.2, 3.3 and 3.6) attenuated wound-induced surface potential changes. Jasmonate-response gene expression in leaves distal to wounds was reduced in a glr3.3 glr3.6 double mutant. This work provides a genetic basis for investigating mechanisms of long-distance wound signalling in plants and indicates that plant genes related to those important for synaptic activity in animals function in organ-to-organ wound signalling.