DETERMINATION OF PHYTOPLANKTON PIGMENTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
DETERMINATION OF PHYTOPLANKTON PIGMENTS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
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DOI：
10.1016/s0003-2670(01)93190-3
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发表时间：
1979-01-01
影响因子：
6.2
通讯作者：
RILEY, JP
中科院分区：
文献类型：
--
作者：
ABAYCHI, JK;RILEY, JP
描述了一种简单高效的液相色谱法测定浮游植物培养物和海洋颗粒物中的叶绿素、叶绿素降解产物和类胡萝卜素。用丙酮和甲醇进行色素提取。在减压下蒸发组合提取物后，颜料在Partisil-10固定相上分离，流动相由轻石油（b.p 60-80°C）、丙酮、二甲基亚砜和二乙胺（体积比为75.23.25:1.5:0.25）组成。当叶绿素存在时，进一步发展为类似的，但更极性的溶剂混合物。分光光度法在440nm处进行检测。该方法对叶绿素的灵敏度约为80 ng，对胡萝卜素的灵敏度约为5 ng。色谱阶段的变异系数在0.6 ~ 1.8%之间。
A simple high-performance liquid Chromatographic method is described for the determination of chlorophylls, chlorophyll degradation products, and carotenoids in phyto-plankton cultures and marine particulate matter. Pigment extraction is carried out with acetone and methanol. After evaporation of the combined extracts under reduced pressure, the pigments are separated on a Partisil-10 stationary phase with a mobile phase consisting of light petroleum (b.p. 60–80°C), acetone, dimethyl sulphoxide and diethylamine(75: 23.25:1.5: 0.25 by volume). When chlorophyllcis present, a further development is performed with a similar, but more polar, solvent mixture. Detection is carried out spectrophotometrically at 440 nm. The method has a sensitivity for the chlorophylls of ca. 80 ng, and for carotene of ca. 5 ng. The coefficient of variation of the Chromatographic stage of the procedure lies in the range 0.6–1.8%.