Insect egg deposition induces defence responses in Pinus sylvestris:: characterisation of the elicitor
Insect egg deposition induces defence responses in Pinus sylvestris:: characterisation of the elicitor
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DOI：
10.1242/jeb.01578
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发表时间：
2005-05-01
影响因子：
2.8
通讯作者：
Mumm, R
中科院分区：
文献类型：
--
作者：
Hilker, M;Stein, C;Mumm, R
植食性松叶蜂DiprionpiniL.的产卵。（Hypltera，Diprionidae）在樟子松（Pinus sylvestris L.）吸引卵寄生蜂红胸姬小蜂（Hyattera，Eulophidae）。卵寄生蜂杀死卵，从而防止因喂养叶蜂幼虫而对植物造成损害。已知诱导松树反应的激发子位于输卵管分泌物中，当雌性叶蜂使用硬化的产卵器阀将卵插入松针中的狭缝中时，雌性叶蜂将输卵管分泌物应用于卵。在这项研究中，我们的特点是诱导子。当从输卵管中分离并直接应用于松针中的狭缝时，激发子仍然有活性。然而，一旦输卵管分泌物溶解在Aqua dest中。在室温或-80 ℃冷冻保存3 h后，其活性丧失。相反，输卵管分泌物保持其激发活性，当溶解在林格溶液（pH 7.2）在室温下储存后，冷冻后。用蛋白酶K处理输卵管分泌物后，激发子的活性消失，蛋白质被破坏。这表明输卵管分泌物中的激发子是一种肽或蛋白质，或者是与这些肽或蛋白质结合的组分。SDS-PAGE显示血淋巴和输卵管分泌物的蛋白质模式相似，但不完全相同。血淋巴本身无诱导作用。输卵管分泌物中的激发子只有在转移到狭缝松针时才有活性，因为它在未受损的针叶上的应用不会诱导有吸引力的挥发物的排放。
Egg deposition by the phytophagous sawfly Diprion pini L. (Hymenoptera, Diprionidae) is known to induce locally and systemically the emission of volatiles in Scots pine (Pinus sylvestris L.) that attract the egg parasitoid Chrysonotomyia ruforum Krausse (Hymenoptera, Eulophidae). The egg parasitoids kill the eggs and thus prevent damage to the plant from feeding sawfly larvae. The elicitor inducing the pine's response is known to be located in the oviduct secretion which the female sawfly applies to the eggs when inserting them into a slit in the pine needle using the sclerotized ovipositor valves. In this study we have characterized the elicitor. The elicitor was still active when isolated from the oviduct and applied directly to slits made in the pine needles. However, as soon as the oviduct secretion was dissolved in Aqua dest. and stored for 3 h at room temperature or kept frozen at -80 degrees C, its activity was lost. In contrast, oviduct secretion kept its eliciting activity, when dissolved in Ringer solution (pH 7.2) both after storage at room temperature and after freezing. The activity of the elicitor vanished after treatment of the oviduct secretion with proteinase K, which destroyed all proteins. This suggests that the elicitor in the oviduct secretion is a peptide or protein, or a component bound to these. SDS-PAGE revealed a similar, but not identical protein pattern from hemolymph and oviduct secretion. Hemolymph itself has no eliciting effect. The elicitor in the oviduct secretion is only active when transferred to slit pine needles, since its application on undamaged needles did not induce the emission of attractive volatiles.