Development of SCAR Markers to Determine the Mating Types of Lepista nuda Protoplast Monokaryons
Development of SCAR Markers to Determine the Mating Types of Lepista nuda Protoplast Monokaryons
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DOI：
10.1007/s00284-013-0510-7
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发表时间：
2014-04-01
影响因子：
2.6
通讯作者：
Xu, Feng
中科院分区：
文献类型：
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作者：
Li, Dengjin;Liu, Yu;Xu, Feng
光叶香蘑Lepista nuda（Bull.ex Fr.）库克属于口蘑科，是一种兼具经济和药用价值的食用菌。菌丝体是从L. nuda和用于制备原生质体单核体。获得了115个单核体，并利用体细胞不亲和性试验确定了它们的交配型。原生质体单核体分为A（1）B（1）或A（2）B（2）交配型。利用简单重复序列和序列相关扩增多态性指纹技术对这些单核体的交配型进行了分析，并开发了16个序列特征的扩增区域引物，以有效地区分单核体的交配型。建立了多重PCR分析方法。本文提供的数据概述了一种精确、快速鉴定原生质体单核交配型的方法，该方法有望缩短常规杂交育种所需的时间。
Lepista nuda (Bull. ex Fr.) Cooke belongs to Tricholomataceae and is an edible fungus with both economic and medical value. Mycelia were isolated from the fruiting bodies of L. nuda and were used to prepare the protoplast monokaryons. One hundred and fifteen monokaryons were obtained and their mating types were determined using somatic incompatibility tests. Protoplast monokaryons segregated into either the A(1)B(1) or the A(2)B(2) mating types. Inter-simple sequence repeats and sequence-related amplified polymorphism fingerprinting were used to analyse the mating types of these protoplast monokaryons and 16 sequence-characterised amplified region primers were developed to efficiently differentiate between the monokaryon mating types. Multiplex PCR analyses were also established. The data presented here outline a method for the precise and rapid identification of protoplast monokaryon mating types, which has the promise to shorten the period required for conventional crossbreeding.