A novel electroporation system for efficient molecular delivery into Chlamydomonas reinhardtii with a 3-dimensional microelectrode.
A novel electroporation system for efficient molecular delivery into Chlamydomonas reinhardtii with a 3-dimensional microelectrode.
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DOI：
10.1038/srep15835
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发表时间：
2015-11-02
影响因子：
4.6
通讯作者：
Kim YC
中科院分区：
文献类型：
--
作者：
Kang S;Kim KH;Kim YC
在众多的转染法中，电穿孔技术以其高效、简便的特点成为应用最广泛的转染法之一。以往的微电穿孔系统存在一些缺点，如高度和设计的限制、耗时和由于技术限制而导致的制造工艺昂贵等。本研究利用3D打印技术制作了三维微电极。用3D打印机注射聚乳酸组成的交指微结构，并用一系列浸渍涂层在银和铝上涂覆。在相同的电场强度(V cm−1)下，微电极比标准试管具有更高的分子传递效率和细胞存活率。此外，本研究还研究了电穿孔过程中的焦耳加热和金属溶解等化学物理变化，结果表明微电穿孔系统的化学物理变化较小。结论是，所提出的微电穿孔系统将有助于基因工程作为一种有前途的递送工具，这种3D打印和电穿孔的组合在不同的设计或系统中具有许多潜在的应用。
Electroporation is one of the most widely used transfection methods because of its high efficiency and convenience among the various transfection methods. Previous micro-electroporation systems have some drawbacks such as limitations in height and design, time-consuming and an expensive fabrication process due to technical constraints. This study fabricates a three dimensional microelectrode using the 3D printing technique. The interdigitated microstructure consisting of poly lactic acid was injected by a 3D printer and coated with silver and aluminum with a series of dip-coatings. With the same strength of electric field (V cm−1), a higher efficiency for molecular delivery and a higher cellular viability are achieved with the microelectrode than with a standard cuvette. In addition, this study investigates chemicophysical changes such as Joule heating and dissolved metal during electroporation and showed the micro-electroporation system had less chemicophysical changes. It was concluded that the proposed micro-electroporation system will contribute to genetic engineering as a promising delivery tool, and this combination of 3D printing and electroporation has many potential applications for diverse designs or systems.