Macrophage subsets harbouring Leishmania donovani in spleens of infected BALB/c mice:: localization and characterization
Macrophage subsets harbouring Leishmania donovani in spleens of infected BALB/c mice:: localization and characterization
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DOI：
10.1046/j.1462-5822.2000.00070.x
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发表时间：
2000-10-01
影响因子：
3.4
通讯作者：
Antoine, JC
中科院分区：
文献类型：
--
作者：
Lang, T;Avé, P;Antoine, JC
本研究的目的是表征位于感染杜氏利什曼原虫的BALB/c小鼠脾脏中的含寄生虫细胞。特别是，这些细胞的MHC II类分子的表达进行了检查，以确定它们是否可以潜在地作为能够免疫刺激利什曼反应性CD 4（+）T淋巴细胞的细胞。为此，对感染后不同时间点采集的脾脏进行免疫组织学分析。使用这种方法，我们观察到，在红髓中，形成小的细胞炎症，含有严重感染的巨噬细胞，可以用单克隆抗体MOMA-2和FA/11染色。它们都表达高水平的MHC II类分子。在白色牙髓中也检测到寄生虫，尤其是在动脉周围淋巴鞘的MOMA-2（+）、FA/11（+）和MHC II类+巨噬细胞以及MOMA-2（+）边缘区巨噬细胞中。在通过粘附富集后，通过荧光显微镜进一步表征感染的细胞。通过该程序回收的所有感染的单核细胞都可以用MOMA-2和FA/11染色，因此很可能属于单核吞噬细胞谱系。此外，无论感染后的时间点如何，它们都强烈表达MHC II类分子和H-2 M分子。共聚焦显微镜分析的寄生虫空泡（PV）表明，这些隔间包围着丰富的溶酶体糖蛋白灯-1和灯-2，在macrosialin（膜蛋白的前溶酶体识别FA/11）和MOMA-2抗原的膜。大约80%的PV的膜上还含有MHC II类和H-2 M分子。总之，这些数据表明，在L。donovani感染的小鼠中，高百分比的无鞭毛体位于表达MHC II类分子的巨噬细胞中，并且它们生活在PV中，表现出与在暴露于低剂量干扰素γ（IFN-γ）并在体外感染的小鼠骨髓来源的巨噬细胞中检测到的PV相似的性质。
The purpose of the current study was to characterize parasite-containing cells located in spleens of BALB/c mice infected with Leishmania donovani. In particular, expression of MHC class II molecules by these cells was examined to determine whether they could potentially act as cells capable of immunostimulating Leishmania-reactive CD4(+) T lymphocytes. To this end, an immunohistological analysis of spleens taken at various time points after infection was undertaken. Using this approach, we observed, in the red pulp, the formation of small cellular infliltrates containing heavily infected macrophages that could be stained with the monoclonal antibodies MOMA-2 and FA/11. All of them expressed high levels of MHC class II molecules. Parasites were also detected in the white pulp, especially in MOMA-2(+), FA/11(+) and MHC class II+ macrophages of the periarteriolar lymphocyte sheath and in MOMA-2(+) marginal zone macrophages. Infected cells were further characterized by fluorescence microscopy after their enrichment by adherence. All infected mononuclear cells recovered by this procedure could be stained with MOMA-2 and FA/11 and thus very probably belonged to the mononuclear phagocyte lineage. Furthermore, all of them strongly expressed both MHC class II as well as H-2M molecules, regardless of the time points after infection. Analysis of the parasitophorous vacuoles (PV) by confocal microscopy showed that these compartments were surrounded by a membrane enriched in lysosomal glycoproteins lamp-1 and lamp-2, in macrosialin (a membrane protein of prelysosomes recognized by FA/11) and in MOMA-2 antigen. About 80% of the PV also had MHC class II and H-2M molecules on their membrane. Altogether, these data indicate that in the spleens of L. donovani-infected mice, a high percentage of amastigotes are located in macrophages expressing MHC class II molecules and that they live in PV exhibiting properties similar to those of PV detected in mouse bone marrow-derived macrophages exposed to a low dose of interferon gamma (IFN-gamma) and infected in vitro.