Studies on the terminal stages of immune hemolysis. V. Evidence that not all complement-produced transmembrane channels are equal.
Studies on the terminal stages of immune hemolysis. V. Evidence that not all complement-produced transmembrane channels are equal.
复制标题
免疫溶血末期的研究。
DOI：
10.4049/jimmunol.123.1.71
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发表时间：
1979
影响因子：
4.4
通讯作者：
Tibor Borsos
中科院分区：
文献类型：
--
作者：
Michael Boyle;Tibor Borsos
研究了 0.1 M EDTA 对 EA 与全 GPC 孵育制备的 E 裂解的抑制作用。在高终点裂解（大于 70%）时，0.1 M EDTA 无法阻止血红蛋白释放，而在低终点（小于 60%），0.1 M EDTA 有效。在所有情况下，25% BSA 都会抑制血红蛋白释放。当通过将EAC1-8与C9一起孵育来制备E时，获得了类似的结果。在该系统中，0.1 M EDTA 在高或低终点裂解时抑制血红蛋白释放的能力差异与低终点裂解不相关，不能与病变/细胞的数量相关，但似乎与 C9 与 SAC1-8 的比率相关。在限制 SAC1-8 和过量 C9 的情况下，产生 E，其中 0.1 M EDTA 不能阻止血红蛋白释放，而在较低的 C9 与 SAC1-8 比例下，0.1 M EDTA 可以阻止血红蛋白释放。这些差异很可能反映了以不同的 C9 与 SAC1-8 比例产生的功能上不同大小的跨膜通道。
The inhibitory effects of 0.1 M EDTA on the lysis of E prepared by incubating EA with whole GPC was studied. At high end point lysis (greater than 70%) 0.1 M EDTA failed to prevent hemoglobin release whereas at lower end point (less than 60%) 0.1 M EDTA was effective. In all cases hemoglobin release was inhibited by 25% BSA. When E were prepared by incubating EAC1-8 with C9, similar results were obtained. In this system the difference in the ability of 0.1 M EDTA to inhibit hemoglobin release at high or low end point lysis could not be correlated with the low end point lysis could not be correlated with the number of lesions/cell but appeared to be related to the C9 to SAC1-8 ratio. With limiting SAC1-8 and excess C9, E were produced from which hemoglobin release could not be prevented by 0.1 M EDTA whereas at lower C9 to SAC1-8 ratios hemoglobin release was prevented by 0.1 M EDTA. These differences most probably reflect functionally different sized transmembrane channels that were produced at different C9 to SAC1-8 ratios.