3,4,5-Tri-O-Caffeoylquinic Acid Promoted Hair Pigmentation Through β-Catenin and Its Target Genes
3,4,5-Tri-O-Caffeoylquinic Acid Promoted Hair Pigmentation Through β-Catenin and Its Target Genes
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DOI：
10.3389/fcell.2020.00175
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发表时间：
2020-03-25
影响因子：
5.5
通讯作者：
Isoda, Hiroko
中科院分区：
文献类型：
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作者：
Bejaoui, Meriem;Villareal, Myra O.;Isoda, Hiroko
毛囊经历了一个由三个阶段组成的规则周期：生长期、退化期和休止期。毛囊黑素细胞的寿命完全与毛发周期有关；在生长期或生长期，黑素细胞活跃并产生黑色素，负责毛干的色素沉积。不同的信号通路调节毛发生长周期，从而调节色素沉着；我们区分Wnt/β-catenin信号通路，因为它在黑素细胞的发育、生长和增殖以及黑素生成酶和相关转录因子的激活中发挥重要作用。在这项研究中，3，4，5-三-O-咖啡酰奎宁酸(TCQA)，咖啡酰奎宁酸的衍生物，刺激了C3H小鼠毛囊、人黑素细胞和B16F10黑色素瘤细胞的色素沉着。在体内和体外观察到TCQA处理后色素沉着相关基因的增强。有趣的是，在处理过的小鼠皮肤和色素细胞系中，β-连环蛋白的表达显著上调。此外，TCQA在抑制人黑素细胞后上调CTNNB1的表达。综上所述，这项研究表明，TCQA触发了β-连环素的激活，以增强头发周期生长期的色素沉着。
The hair follicle undergoes a regular cycle composed of three phases: anagen, catagen, and telogen. The life of follicular melanocytes is totally linked to the hair cycle; and during anagen or the growth phase, the melanocytes are active and produce the melanin responsible of hair shaft pigmentation. Various signaling pathways regulate the hair growth cycle and, therefore, the pigmentation; we distinguish the Wnt/beta-catenin signaling pathway as it plays a major role in the development, growth, and proliferation of the melanocytes and the activation of melanogenesis enzymes and the related transcription factor. In this study, 3,4,5-tri-O-caffeoylquinic acid (TCQA), a caffeoylquinic acid derivative, stimulated the pigmentation in C3H mouse hair follicle, in human melanocytes, and B16F10 melanoma cells. An enhancement in pigmentation associated genes was observed upon TCQA treatment in vivo and in vitro. Interestingly, the expression of beta-catenin was remarkably upregulated in mouse treated skin and in pigment cell lines. Moreover, TCQA upregulated CTNNB1 expression after inhibition in human melanocytes. Taken together, this study suggests that TCQA triggered beta-catenin activation to enhance the pigmentation during the anagen phase of the hair cycle.