Tobamovirus-resistant tobacco generated by RNA interference directed against host genes
Tobamovirus-resistant tobacco generated by RNA interference directed against host genes
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DOI：
10.1016/j.febslet.2005.07.021
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发表时间：
2005-08-15
期刊：
影响因子：
3.5
通讯作者：
Ishikawa, M
中科院分区：
文献类型：
--
作者：
Asano, M;Satoh, R;Ishikawa, M
已鉴定出两个同源的烟草基因NtTOM 1和NtTOM 3。这些基因编码与拟南芥TOM1和TOM3具有氨基酸序列相似性的多肽，其平行发挥功能以支持病毒增殖。同时RNA干扰N.结果表明，烟草花叶病毒对番茄花叶病毒和其它黄瓜花叶病毒的增殖几乎完全抑制，但不影响植物生长或黄瓜花叶病毒的增殖能力。由于TOM1和TOM3同源物存在于多种植物物种中，通过RNA干扰抑制它们应该构成用于产生抗番茄花叶病毒植物的有用方法。(c)2005年欧洲生物化学学会联合会。Elsevier B.V.出版，保留所有权利。
Two homologous Nicotiana tabacum genes NtTOM1 and NtTOM3 have been identified. These genes encode polypeptides with amino acid sequence similarity to Arabidopsis thaliana TOMI and TOM3, which function in parallel to support tobamovirus multiplication. Simultaneous RNA interference against NtTOM1 and NtTOM3 in N. tabacum resulted in nearly complete inhibition of the multiplication of Tomato mosaic virus and other tobamoviruses, but did not affect plant growth or the ability of Cucumber mosaic virus to multiply. As TOM1 and TOM3 homologues are present in a variety of plant species, their inhibition via RNA interference should constitute a useful method for generating tobamovirus-resistant plants. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.