Transepithelial electrical resistance (TEER): a functional parameter to monitor the quality of oviduct epithelial cells cultured on filter supports.
Transepithelial electrical resistance (TEER): a functional parameter to monitor the quality of oviduct epithelial cells cultured on filter supports.
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DOI：
10.1007/s00418-015-1351-1
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发表时间：
2015-11
影响因子：
2.3
通讯作者：
Schoen J
中科院分区：
文献类型：
--
作者：
Chen S;Einspanier R;Schoen J
在多孔过滤器上培养输卵管上皮细胞可培养出与活体相似的形态和功能。然而，由于滤光材料的光学特性和细胞的柱状形状，细胞的质量很难通过光学显微镜来评估。在这项研究中，我们的目的是评估经上皮电阻（TEER）测量作为猪输卵管上皮细胞（POEC）培养的预后质量指标。POEC在四种不同类型的培养基中保持3和6 w，以获得不同的培养质量，并在处理样品进行组织学处理之前测量TEER。使用形态学标准（纤毛的存在，融合和细胞极性）对培养质量进行评分。我们进一步分析了在完全分化的常规培养（生物变异）和由于激素刺激而改变细胞高度的培养中，细胞高度（作为顶基极化的量度）与TEER之间的相关性。完全分化的培养具有中等TEER，在500和1100 Ω*cm2之间。只有5%的培养物显示TEER值在这个定义范围内，质量差。亚分化培养表现为极低或过高的TEER。我们发现，在高分化培养（常规和激素刺激组）中，TEER和上皮高度之间存在高度显著（P < 0.0001）负线性相关。这可能表明紧密连接组装与上皮的顶基极化之间的相互作用。综上所述，TEER是一种直观的质量指标，可常规用于体外监测输卵管上皮细胞的分化状态。本文的在线版本（doi:10.1007/s00418-015-1351-1）包含补充材料，仅供授权用户使用。
Cultivation of oviduct epithelial cells on porous filters fosters in vivo-like morphology and functionality. However, due to the optical properties of the filter materials and the cells’ columnar shape, cell quality is hard to assess via light microscopy. In this study, we aim to evaluate transepithelial electrical resistance (TEER) measurement as a prognostic quality indicator for the cultivation of porcine oviduct epithelial cells (POEC). POEC were maintained in four different types of media for 3 and 6 w to achieve diverse culture qualities, and TEER was measured before processing samples for histology. Culture quality was scored using morphological criteria (presence of cilia, confluence and cell polarity). We furthermore analyzed the correlation between cellular height (as a measure of apical–basal polarization) and TEER in fully differentiated routine cultures (biological variation) and in cultures with altered cellular height due to hormonal stimulation. Fully differentiated cultures possessed a moderate TEER between 500 and 1100 Ω*cm2. Only 5 % of cultures which exhibited TEER values in this defined range had poor quality. Sub-differentiated cultures showed either very low or excessively high TEER. We unveiled a highly significant (P < 0.0001) negative linear correlation between TEER and epithelial height in well-differentiated cultures (both routine and hormone stimulated group). This may point toward the interaction between tight junction assembly and epithelial apical–basal polarization. In conclusion, TEER is a straightforward quality indicator which could be routinely used to monitor the differentiation status of oviduct epithelial cells in vitro. The online version of this article (doi:10.1007/s00418-015-1351-1) contains supplementary material, which is available to authorized users.