Fabrication and characterization of hydroxyapatite/sodium alginate/chitosan composite microspheres for drug delivery and bone tissue engineering
Fabrication and characterization of hydroxyapatite/sodium alginate/chitosan composite microspheres for drug delivery and bone tissue engineering
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DOI：
10.1016/j.msec.2019.03.040
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发表时间：
2019-07-01
影响因子：
7.9
通讯作者：
Deng, Shi-ting
中科院分区：
文献类型：
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作者：
Bi, Yong-guang;Lin, Zi-ting;Deng, Shi-ting
采用乳液交联技术制备了具有良好生物相容性的羟基磷灰石/海藻酸钠/壳聚糖（HA/SA/CS）复合微球；用钙离子作为交联剂。考察了海藻酸钠（SA）浓度、水油体积比、羟基磷灰石（HA）纳米颗粒含量以及旋转速度对复合微球形貌和分散性的影响。考察了材料的载药、释药行为、体外溶血活性、细胞毒性、细胞粘附和增殖能力。结果表明：成功制备了HA/SA/CS复合微球；其载药量和包封效率远高于透明质酸纳米颗粒。负载dox的HA/SA/CS复合微球具有良好的ph敏感释药能力。溶血和细胞毒性试验表明，微球具有良好的血液和细胞相容性。此外，制备的复合微球比HA纳米颗粒和HA/SA复合微球具有更好的细胞粘附和增殖能力。因此，HA/SA/CS复合微球可能具有作为ph响应型控释药物递送系统的药物载体和骨组织工程应用的候选材料的潜力。
Hydroxyapatite/sodium alginate/chitosan (HA/SA/CS) composite microspheres, which possess good biocompatibility for specific biomedical application, were prepared using an emulsion crosslink technique; calcium ions were used as a cross-linking agent. The effect of the concentration of sodium alginate (SA), the volume ratio of water to oil, the content of hydroxyapatite (HA) nanoparticles, as well as rotation speed, on the morphology and dispersion of composite microspheres were investigated. Also investigated were the drug loading, release behaviors, in vitro hemolysis activity, cytotoxicity, cell adhesion and proliferation capacity of the materials. The results demonstrate that the HA/SA/CS composite microspheres were successfully prepared; their drug loading and encapsulation efficiency are much higher than that of HA nanoparticles. Dox-loaded HA/SA/CS composite microspheres show good pH-sensitive drug-release capability. The hemolysis and cytotoxicity tests suggest that the microspheres have good blood and cell compatibility. Furthermore, the prepared composite microspheres display better cell adhesion and proliferation capacity than HA nanoparticles and HA/SA composite microspheres. Therefore, the HA/SA/CS composite microspheres might have potential as drug carriers in a pH-responsive controlled-release drug delivery system and as candidates for application in bone tissue engineering.