Induction of interleukin-8 messenger RNA in heart and skeletal muscle during pediatric cardiopulmonary bypass.
Induction of interleukin-8 messenger RNA in heart and skeletal muscle during pediatric cardiopulmonary bypass.
复制标题
小儿体外循环期间心脏和骨骼肌中白细胞介素 8 信使 RNA 的诱导。
DOI：
10.1161/01.cir.92.9.315
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发表时间：
1995
期刊：
影响因子：
37.8
通讯作者：
Neufeld,EJ
中科院分区：
文献类型：
--
作者：
Burns,SA;Newburger,JW;Xiao,M;MayerJr,JE;Walsh,AZ;Neufeld,EJ
白细胞介素-8 （IL-8）是主要的中性粒细胞趋化因子，通过激活中性粒细胞并促进其向组织迁移来促进炎症组织损伤。体外循环（CPB）患者血清IL-8水平升高。本研究的目的是确定IL-8基因表达是否在有或没有低温骤停的CPB组织中被激活。方法和结果采用核糖核酸酶保护法检测小儿先天性心脏缺损CPB修复前后人心房和骨骼肌样本中sil -8转录水平。用磷成像仪对结果进行定量分析。16例患者中有14例在CPB期间心房IL-8 mRNA水平升高（中位升高，2.9倍；P= 0.0029）。在骨骼肌中，12例患者中有11例IL-8 mRNA升高（中位数，12倍；P= 0.012）。心房和肌肉IL-8诱导程度与总支撑时间和交叉钳夹时间无直接关系。骨骼肌转录增加发生在有或没有循环停止的情况下，表明CPB单独刺激足以诱导信息产生。在心房和骨骼肌患者中IL-8 mRNA的基线值差异很大。原位杂交分析显示，CPB后组织中IL-8 mRNA弥漫性升高，在一些小静脉中明显升高。结论：大多数CPB患者心肌和骨骼肌均产生IL-8 mRNA。这可能导致局部IL-8浓度升高，导致CPB后组织损伤。
BackgroundInterleukin-8 (IL-8), the major neutrophil chemoattractant factor, contributes to inflammatory tissue injury by activating neutrophils and promoting their migration into tissue. IL-8 levels increase in serum of patients undergoing cardiopulmonary bypass (CPB). The purpose of this study was to determine if IL-8 gene expression is activated in tissues subjected to CPB with or without hypothermic arrest.Methods and ResultsIL-8 transcript levels were measured by ribonuclease protection in samples of human atrium and skeletal muscle from children before and after CPB for repair of congenital heart defects. Results were quantified by PhosphorImager. Atrial IL-8 mRNA levels increased during CPB in 14 of 16 patients tested (median increase, 2.9-fold;P=.0029). In skeletal muscle, IL-8 mRNA increased in 11 of 12 patients (median, 12-fold;P=.012). Degree of IL-8 induction in atrium and muscle was not directly associated with total support time or cross-clamp time. Transcript increase in skeletal muscle occurred with or without a period of circulatory arrest, suggesting that the stimulus of CPB alone was sufficient to induce message production. Baseline values for IL-8 mRNA varied widely among patients in atrium and skeletal muscle. In situ hybridization analysis revealed diffuse increase in IL-8 mRNA throughout the tissue after CPB, with striking increase in some small veins.ConclusionsWe conclude that production of IL-8 mRNA occurs in most patients during CPB in both myocardium and skeletal muscle. This may result in high local IL-8 concentrations, contributing to the tissue injury after CPB.