Ethanol-induced iNOS and COX-2 expression in cultured astrocytes via NF-κB
Ethanol-induced iNOS and COX-2 expression in cultured astrocytes via NF-κB
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DOI：
10.1097/00001756-200403220-00021
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发表时间：
2004-03-22
期刊：
影响因子：
1.7
通讯作者：
Guerri, C
中科院分区：
文献类型：
--
作者：
Blanco, AM;Pascual, M;Guerri, C
中枢神经系统特别容易受到酒精和毒性的影响。星形胶质细胞是免疫活性细胞，这些细胞的活化与几种神经退行性疾病有关。通过使用培养的皮质星形胶质细胞，我们表明，短的乙醇处理（100 mM）能够上调环氧合酶2（考克斯-2）和诱导型一氧化氮合酶（iNOS）的表达，并且这些效果是通过核因子κ B（NF-κ B）调节，如通过吡咯烷二硫代氨基甲酸酯（PDTC）或BAY 11-7082抑制NF-κ B激活所揭示的。这些结果表明，乙醇能够通过NF-κ B活化诱导星形胶质细胞中的炎症介质。
The CNS is particularly susceptible to the effects of alcohol and toxicity. Astrocytes are immunoactive cells, and the activation of these cells is associated with several neurodegenerative disorders. By using cultured cortical astrocytes, we show that a short ethanol treatment (100 mM) is able to up-regulate both cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) expression, and that these effects are regulated via nuclear factor kappa B (NF-kappaB) as revealed by the inhibition of NF-kappaB activation with pyrrolidine dithiocarbamate (PDTC) or BAY 11-7082. These results suggest that ethanol is able to induce inflammatory mediators in astrocytes through the NF-kappaB activation.