THE DETECTION OF ENDOTOXIN BY INVITRO PRODUCTION OF ENDOGENOUS PYROGEN - COMPARISON WITH LIMULUS AMEBOCYTE LYSATE GELATION
THE DETECTION OF ENDOTOXIN BY INVITRO PRODUCTION OF ENDOGENOUS PYROGEN - COMPARISON WITH LIMULUS AMEBOCYTE LYSATE GELATION
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DOI：
10.1016/0022-1759(82)90004-7
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发表时间：
1982-01-01
影响因子：
2.2
通讯作者：
ATKINS, E
中科院分区：
文献类型：
--
作者：
DUFF, GW;ATKINS, E
本文测定了白细胞内源性热原（EP）产生和鲎变形细胞溶解物（LAL）凝胶对大肠杆菌内毒素（最小致热原剂量为4 ng/kg）的敏感性。LAL可检测到0.5-1.0 ng/ml的浓度。产生可检测EP的最小内毒素浓度为2 × 10 - 6 μ g/ml。106单核细胞低10倍（0.05-0.1 ng/ml）。在0.4ng/ml的内毒素浓度下，可检测的EP产生所需的单核细胞的最小数目为5x。105.因此，LAL凝胶试验不能安全地用于排除测定EP产生的细胞系统中的显著内毒素污染。同样的结论甚至更有力地适用于淋巴细胞活化因子（LAF，白细胞介素1）的体外生产，因为LAF和EP似乎是相同的，并且在LAF试验中很容易检测到亚热原量的EP。
The sensitivities of leukocyte endogenous pyrogen (EP) production and Limulus amebocyte lysate (LAL) gelation to endotoxin from Escherichia coli (minimum i.v. pyrogenic dose, 4 ng/kg in rabbits) were determined. Concentrations of 0.5-1.0 ng/ml could be detected by LAL. The minimum endotoxin concentration which genrated detectable EP from 2 .times. 106 monocytes was 10-fold lower (0.05-0.1 ng/ml). At an endotoxin concentration of 0.4 ng/ml, the minimum number of monocytes required for detectable EP production was 5 .times. 105. Thus, the LAL gelation test cannot safely be used to exclude significant endotoxin contamination in a cellular system where EP production is being measured. The same conclusion applies even more forcibly to the in vitro production of lymphocyte activating factor (LAF, interleukin 1), since it appears that LAF and EP are identical and sub-pyrogenic amounts of EP are easily detectable in the LAF assay.