Cholesterol-dependent infection of Burkitt's lymphoma cell lines by Epstein-Barr virus
Cholesterol-dependent infection of Burkitt's lymphoma cell lines by Epstein-Barr virus
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DOI：
10.1099/vir.0.19252-0
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发表时间：
2003-11-01
影响因子：
3.8
通讯作者：
Longnecker, R
中科院分区：
文献类型：
--
作者：
Katzman, RB;Longnecker, R
EB病毒（EBV）感染是一个多步骤的过程，首先需要病毒与宿主细胞结合，然后病毒包膜与宿主细胞质膜融合。EBV有效进入B细胞至少需要EBV编码的糖蛋白gp 350与细胞CD 21和gp 42与MHC II类蛋白的相互作用。在这项研究中，使用胆固醇结合药物甲基-β-环糊精和制霉菌素有效地抑制了靶伯基特淋巴瘤B细胞系的EBV感染，表明胆固醇的重要作用，并表明脂筏参与EBV感染。
Epstein-Barr virus (EBV) infection is a multi-step process, first requiring virus binding to the host cell, followed by fusion of the viral envelope with the host cell plasma membrane. Efficient EBV entry into B cells requires, at the minimum, the interaction of the EBV-encoded glycoproteins gp350 with cellular CD21 and gp42 with MHC class II proteins. In this study, use of the cholesterol-binding drugs methyl-beta-cyclodextrin and nystatin efficiently inhibited EBV infection of target Burkitt's lymphoma B-cell lines, indicating an important role for cholesterol and suggesting the involvement of lipid rafts in EBV infection.