Low-level HIV infection of hepatocytes.
Low-level HIV infection of hepatocytes.
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DOI：
10.1186/1743-422x-9-157
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发表时间：
2012-08-09
期刊：
影响因子：
4.8
通讯作者：
Blackard JT
中科院分区：
文献类型：
--
作者：
Kong L;Cardona Maya W;Moreno-Fernandez ME;Ma G;Shata MT;Sherman KE;Chougnet C;Blackard JT
关于HIV感染是否发生在肝脏内的数据有限;因此，我们在两种肝细胞系- Huh7.5和Huh7.5JFH 1-以及原代人肝细胞中探索了HIV生命周期的早期和晚期阶段。在Huh7.5和Huh7.5JFH 1细胞以及原代肝细胞中检测到整合的HIV DNA，并以剂量依赖性方式被整合酶抑制剂雷特格韦抑制。在感染后第1、3、5和7天的细胞培养上清液中也检测到HIV p24蛋白，并被AZT抑制，尽管水平与淋巴细胞系中的水平相比是适度的。HIV感染肝细胞的培养上清液能够感染非肝HIV指示细胞系。这些结果表明，低水平的HIV复制在体外肝细胞补充证据表明，HIV在体内对肝脏有害的影响。
There are only limited data on whether HIV infection occurs within the liver; therefore, we explored early and late stages of the HIV life cycle in two hepatocyte cell lines – Huh7.5 and Huh7.5JFH1 – as well as in primary human hepatocytes. Integrated HIV DNA was detected in Huh7.5 and Huh7.5JFH1 cells, as well as in primary hepatocytes, and was inhibited by the integrase inhibitor raltegravir in a dose-dependent manner. HIV p24 protein was also detected in cell culture supernatants at days 1, 3, 5, and 7 post-infection and was inhibited by AZT, although levels were modest compared to those in a lymphocyte cell line. Culture supernatants from HIV-infected hepatocytes were capable of infecting a non-hepatic HIV indicator cell line. These results indicating low-level HIV replication in hepatoctyes in vitro complement evidence suggesting that HIV has deleterious effects on the liver in vivo.