Effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokines and the subsequent recruitment of immunocompetent cells in the oviduct of laying and molting hens
Effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokines and the subsequent recruitment of immunocompetent cells in the oviduct of laying and molting hens
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DOI：
10.3382/ps.2011-01596
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发表时间：
2011-10-01
期刊：
影响因子：
4.4
通讯作者：
Yoshimura, Y.
中科院分区：
文献类型：
--
作者：
Nii, T.;Sonoda, Y.;Yoshimura, Y.
本研究的目的是检查脂多糖 (LPS) 是否诱导促炎细胞因子和趋化因子的表达并在输卵管下部招募 T 细胞，以及产蛋期和蜕皮期对 LPS 的反应是否不同。白来亨蛋鸡和换毛母鸡静脉注射盐水（对照）或LPS。注射后3或6小时收集子宫和阴道输卵管，用于IL-1β、IL-6、IL-8(CXCLi2)和淋巴趋化素(Lptn)的逆转录PCR分析，并用于CD4+和CD8+T细胞频率的免疫组化分析。蛋鸡和换羽母鸡注射 LPS 后 3 或 6 小时，子宫中 IL-1β、IL-6 和 CXCLi2 的表达以及阴道中 IL-1β 的表达均上调。在蛋鸡中，LPS 上调了阴道中 CXCLi2 的表达，而在换羽母鸡中，LPS 的影响并不显着。 Lptn表达在注射LPS 3小时后呈下调趋势，注射LPS后6小时恢复。 6 小时后，产蛋鸡和换羽母鸡子宫和阴道固有层中 CD4+ T 细胞的频率趋于增加，以响应 LPS。 6 小时后，蛋鸡子宫和阴道固有层中的 CD8+ T 细胞频率因 LPS 的反应而增加。然而，在换羽母鸡中，LPS 刺激仅导致阴道中的 CD8+ T 细胞增加，而子宫中则没有。这些结果表明促炎细胞因子和 CXCLi2 趋化因子的表达上调与输卵管下部响应 LPS 的 T 细胞招募相关，尽管子宫中的 CD8+ T 细胞在蜕皮阶段可能会受到抑制。这些免疫反应可能在防御输卵管感染方面发挥作用。
The goal of this study was to examine whether lipopolysaccharide (LPS) induces the expression of proinflammatory cytokines and chemokines and recruits T cells in the lower part of the oviduct, and whether that response to LPS is different between the laying and molting phase. White Leghorn laying and molting hens were intravenously injected with saline (control) or LPS. The uterus and vagina of oviducts were collected 3 or 6 h after injection, and used for reverse transcription PCR analysis of IL-1 beta, IL-6, IL-8 (CXCLi2), and lymphotactin (Lptn), and for immunohistochemical analysis for the frequency of CD4+ and CD8+ T cells. The expressions of IL-1 beta, IL-6, and CXCLi2 in the uterus and that of IL-1 beta in the vagina were upregulated in response to LPS 3 or 6 h after injection in both laying and molting hens. The CXCLi2 expression in the vagina was upregulated by LPS in laying hens, whereas those effects of LPS were not significant in molting hens. Expression of Lptn showed a tendency to be downregulated after 3 h, with recovery by 6 h after LPS injection. The frequency of CD4+ T cells tended to increase in response to LPS after 6 h in the lamina propria of the uterus and vagina in both laying and molting hens. The CD8+ T cell frequencies in the lamina propria of the uterus and vagina of laying hens increased in response to LPS after 6 h. However, in the molting hens, LPS stimulation resulted in CD8+ T cell increase in the vagina only and not in the uterus. These results suggest that expressions of proinflammatory cytokines and CXCLi2 chemokine are upregulated in association with T cell recruitment in response to LPS in the lower part of the oviduct, although CD8+ T cells in the uterus may be depressed during the molting phase. These immunoresponses may play roles in the defense against infection of the oviduct.