Femoral osteocyte lacunar density, volume and morphology in women across the lifespan
Femoral osteocyte lacunar density, volume and morphology in women across the lifespan
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DOI：
10.1016/j.jsb.2013.07.004
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发表时间：
2013-09-01
影响因子：
3
通讯作者：
Cooper, David M. L.
中科院分区：
文献类型：
--
作者：
Carter, Yasmin;Thomas, C. David L.;Cooper, David M. L.
骨细胞被认为是骨中机械敏感的主要媒介。由于骨细胞在骨的结构-功能关系中的重要作用，骨细胞及其占据的空间（骨陷窝）越来越受到关注。骨陷窝随年龄的变化在女性中特别受关注，因为她们更容易发生与衰老疾病（包括骨质疏松症）相关的骨质流失和脆性。本研究的目的是测试在人类寿命期间，成年女性的皮质中是否存在腔隙密度（腔隙/mm（3）骨）、方向和形态学差异。采用同步辐射显微CT对30例年龄在20-86岁的女性股骨干前部骨块进行成像。骨陷窝密度与年龄无明显关系。随着年龄的增长，观察到腔隙体积显著减少（p < 0.001），同时观察到腔隙形态的变化。当分为两组（50岁）时，年轻组的骨陷窝相似，大30%，更平坦（p < 0.001），更不等长（球形）（p < 0.001）。据我们所知，观察到的陷窝体积和形态的变化，在人类的寿命是新的，可能是由于优先表面填充细胞外空间。这种填充的功能影响尚不清楚，但这种规模的变化可能会影响骨细胞网络的机械传感功能。分辨率的限制使我们无法评估这种填充是否与泪小管断裂有关。这一假设值得进一步研究，因为如果得到证实，它将对骨细胞网络产生深远的负面影响，并可能为年龄相关的骨丢失提供新的见解。(C)2013 Elsevier Inc. All rights reserved.
Osteocytes are believed to be the primary agents of mechanosensing in bone. Due to this important role in the structure-function relationship of bone, osteocytes and the spaces they occupy (lacunae) are of increasing interest. Changes in lacunae with age are of particular interest in women since they are more susceptible to bone loss and fragility associated with senescent diseases including osteoporosis. This study's purpose was to test whether differences exist in lacunar density (lacunae/mm(3) of bone), orientation and morphology in the cortex of adult women spanning the human lifespan. Anterior blocks from the femoral shaft from 30 women aged 20-86 years were imaged by synchrotron-radiation micro-CT. No significant relation between lacunar density and age was detected. A significant reduction in lacunar volume with age (p < 0.001) was observed, alongside changes in lacunar morphology. When divided into two groups (50 years) the, younger group's lacunae were similar to 30% larger and were flatter (p < 0.001) and less equant (spherical) (p < 0.001). To our knowledge the observation that lacunar volume and morphology change over the human lifespan is novel, potentially resulting from preferential surface infilling within the extracellular space. The functional impact of this infilling is unclear but such a change in scale likely impacts the mechanosensing function of the osteocyte network. Limitations in resolution prevented us from assessing if this infilling is associated with disruption of the canaliculi. This hypothesis warrants further investigation as, if confirmed, it would represent a profound negative impact on the osteocyte network and may provide new insights into age-related bone loss. (C) 2013 Elsevier Inc. All rights reserved.