LOCALIZATION OF ACETYLCHOLINESTERASE POSITIVE NEURONS AND SUBSTANCE-P AND ENKEPHALIN POSITIVE FIBERS BY HISTOCHEMISTRY AND IMMUNOHISTOCHEMISTRY IN THE SYMPATHETIC INTERMEDIATE ZONE OF THE DEVELOPING HUMAN SPINAL-CORD
LOCALIZATION OF ACETYLCHOLINESTERASE POSITIVE NEURONS AND SUBSTANCE-P AND ENKEPHALIN POSITIVE FIBERS BY HISTOCHEMISTRY AND IMMUNOHISTOCHEMISTRY IN THE SYMPATHETIC INTERMEDIATE ZONE OF THE DEVELOPING HUMAN SPINAL-CORD
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DOI：
10.1016/0306-4522(90)90224-r
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发表时间：
1990-01-01
期刊：
影响因子：
3.3
通讯作者：
YEW, DT
中科院分区：
文献类型：
--
作者：
LUO, CB;ZHENG, DR;YEW, DT
采用组织化学和免疫组织化学方法研究了39例妊娠期5 ~ 40周人胚胎/胎儿脊髓中间交感带乙酰胆碱酯酶阳性神经元及P物质和脑啡肽纤维的定位。早在妊娠第5周，中外侧主部核就出现了乙酰胆碱酯酶阳性神经元。妊娠第9 ~ 13周时，索旁中间核、棘间核和室管膜旁隔核也可见阳性神经元。直到足月，这些乙酰胆碱酯酶阳性神经元的数量都有所增加。妊娠第8周时，在主部中间外侧核中首次观察到P物质和脑啡肽纤维，妊娠第14周时，在中间索部核和棘间核中检测到阳性纤维。妊娠第26周时，大核主旁正中肌、索旁正中肌、棘间肌和室管膜旁正中肌均含有P物质和脑啡肽纤维。初步显示的乙酰胆碱酯酶阳性神经元似乎比我们的P物质和脑啡肽阳性纤维更早。
Localization of acetylcholinesterase positive neurons and substance P and enkephalin fibers were studied by histochemistry and immunohistochemistry in the intermediate sympathetic zone of the spinal cords of 39 human embryos/fetuses from gestation ages five to 40 weeks. Acetylcholinesterase of positive neurons were observed in the nucleus intermediolateralis pars principalis as early as the fifth week of gestation. By the ninth to 13 the weeks of gestation, positive neurons were also seen in the nuclei intermedialis pars funicularis, intercalatus spinalis and intercalatus pars paraependymalis. Increase in amount of these positive acetlycholinesterase neurons was demonstrated till term. Substance P and enkephalin fibers were initially observed by the eighth gestation week in the intermediolaterlis pars principalis nucleus and positive fibers were then detected in the nucleus intermediate pars funicularis as well as the nucleus intercalatus spinalis by the 14th week of gestation. By the 26th week of gestation, all the major nuclei intermediolateralis par principalis, intermedialis pars funicularis, intercalatus spinalis and intercalatus pars paraependymalis has substance P and enkephalin fibers. Initial demonstrated of acetylcholinesterase positive neurons appeared to be at an earlier stage than that of our substance P and enkephalin positive fibers.