Image intersification applied to biological problems
Image intersification applied to biological problems
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图像交叉应用于生物问题
DOI：
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发表时间：
1972
期刊：
影响因子：
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通讯作者：
George T. Reynolds
中科院分区：
文献类型：
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作者：
George T. Reynolds
在生物研究的许多重要观察类型中，样本提供的信息以可见光、紫外线或 X 射线的光子量子的形式存在。观察的过程变成了以尽可能高的效率以有用的形式记录这些信息的过程。当由于某种原因，样本提供的量子总数或比率很小时，这个问题就变得尤为重要。此类限制的示例如下： (i) 仅允许低强度照明的过程，以免干扰观察下的生物过程。 (ii) 流程变化非常快并且需要快速序列记录。 (iii) 每个事件仅提供有限数量的光子的过程，例如生物发光。 (iv) 使用放射性示踪剂的过程，并且需要在低比活度或短时间间隔内观察放射性。 (v) X 射线衍射过程，其中样品衍射较弱或 X 射线强度必须保持较低，以免损坏样品。 (vi) 涉及荧光观察的过程，由于标记材料数量的限制，荧光强度较低。
In many important types of observations in biological research, the information provided by the specimens is in the form of photons-quanta of visible light, u.v., or X-rays. The process of observation becomes one of recording this information in useful form, with as high an efficiency as possible. The problem becomes particularly important when for some reason or other the total number or rate of quanta provided by the specimen is small. Examples of such limitations are included in the following: (i) Processes permitting only low-intensity illumination in order not to interfere with the biological processes under observation. (ii) Processes changing very rapidly and requiring rapid sequence recording. (iii) Processes providing only a limited number of photons per event, such as bioluminescence. (iv) Processes in which radioactive tracers are utilized, and observation of radioactivity is desirable at low specific activity or within short time intervals. (v) X-ray diffraction processes where the specimen is weakly diffracting or where the X-ray intensity must be kept low in order not to damage the specimen. (vi) Processes involving the observation of fluorescence, where the intensity is low because of limitations on the amount of tagging material.