Microbiome and Metabolome Analyses Reveal the Disruption of Lipid Metabolism in Systemic Lupus Erythematosus
Microbiome and Metabolome Analyses Reveal the Disruption of Lipid Metabolism in Systemic Lupus Erythematosus
复制标题
微生物组和代谢组分析揭示系统性红斑狼疮脂质代谢的破坏
DOI：
10.3389/fimmu.2020.01703
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发表时间：
2020-07-31
影响因子：
7.3
通讯作者：
Dai, Yong
中科院分区：
文献类型：
--
作者：
He, Jingquan;Chan, Tianlong;Dai, Yong
系统性红斑狼疮（SLE）是一种全身性自身免疫性疾病，影响全世界成千上万的人。最近，代谢和肠道微生物组的改变已成为SLE发病机制的关键调节因子。但肠道菌群与SLE代谢的协调作用尚不清楚。在这里，通过整合16S测序和代谢组学数据，我们表征了SLE患者的肠道微生物组以及粪便和血清代谢组学改变。微生物多样性测序显示SLE患者肠道菌群失调，β多样性显著增加。代谢组学分析在SLE患者的血清和粪便样品中鉴定出43种和55种显著改变的代谢物。值得注意的是，脂质占血清中代谢物改变的约65%，突出了脂质代谢的破坏。综合相关性分析提供了SLE患者肠道微生物组与脂质代谢之间的联系，特别是根据调节初级胆汁酸向次级胆汁酸的转化。总的来说，我们的研究结果说明了SLE患者肠道微生物组和代谢组的扰动，这可能有助于开发新的SLE干预措施。
Systemic lupus erythematosus (SLE) is a systemic autoimmune disease that affects thousands of people worldwide. Recently, alterations in metabolism and gut microbiome have emerged as key regulators of SLE pathogenesis. However, it is not clear about the coordination of gut commensal bacteria and SLE metabolism. Here, by integrating 16S sequencing and metabolomics data, we characterized the gut microbiome and fecal and serum metabolome alterations in patients with SLE. Microbial diversity sequencing revealed gut microflora dysbiosis in SLE patients with significantly increased beta diversity. The metabolomics profiling identified 43 and 55 significantly changed metabolites in serum and feces samples in SLE patients. Notably, lipids accounted for about 65% altered metabolites in serum, highlighted the disruption of lipid metabolism. Integrated correlation analysis provided a link between the gut microbiome and lipid metabolism in patients with SLE, particularly according to regulate the conversion of primary bile acids to secondary bile acids. Overall, our results illustrate the perturbation of the gut microbiome and metabolome in SLE patients which may facilitate the development of new SLE interventions.