EFFECT OF WHITE-LIGHT ON TAXOL AND BACCATIN-III ACCUMULATION IN CELL-CULTURES OF TAXUS-CUSPIDATA SIEB AND ZUCC
EFFECT OF WHITE-LIGHT ON TAXOL AND BACCATIN-III ACCUMULATION IN CELL-CULTURES OF TAXUS-CUSPIDATA SIEB AND ZUCC
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DOI：
10.1016/s0176-1617(11)81918-8
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发表时间：
1995-09-01
影响因子：
4.3
通讯作者：
DICOSMO, F
中科院分区：
文献类型：
--
作者：
FETTNETO, AG;PENNINGTON, JJ;DICOSMO, F
紫杉醇是衍生自红豆杉属（Taxus spp.）并在美国被批准用于治疗卵巢癌和乳腺癌。东北红豆杉细胞培养物代表了紫杉醇和相关紫杉烷的替代来源。我们先前已经描述了T.尖齿的研究了白色光对红豆杉愈伤组织和悬浮细胞中紫杉醇和浆果赤霉素III积累的影响。尖齿的结果表明，紫杉醇和浆果赤霉素III的积累约高出黑暗生长的愈伤组织的三倍。黑暗生长的悬浮液也显示三倍以上的总体积紫杉醇相对于光生长的悬浮液;浆果赤霉素III的量在黑暗和光生长的悬浮液相似。悬浮液的生长在黑暗中高出两到三倍。在黑暗生长的细胞中，紫杉醇积累在稳定期（d27至d45）达到峰值（14 mg. kg（-1）edw）。浆果赤霉素III积累的峰值（180 mg . kg（-1）EDW）出现在紫杉醇高峰之前（d18 ~ d27），且随着紫杉醇浓度的增加，紫杉醇含量下降。在黑暗和光照生长的悬浮液中，培养基中的巴卡亭III积累相似（峰值为6.2 mg . L（-1）d9）。培养基中紫杉醇的积累在黑暗中较高（0.3 mg . L（-1），光照组（0.08 mg . L（-1）d9）。紫杉醇稳态量的下调可能与光对微粒体3-羟基-3-甲基戊二酰辅酶A还原酶和甲羟戊酸供应的影响有关;该酶在光生长细胞中的活性显著降低。
Taxol is a diterpene amide derived from Taxus spp. and approved for the treatment of ovarian and breast cancer in the USA. Taxus cuspidata cell cultures represent an alternate source of taxol and related taxoids. We have previously described the development, growth, and taxoid accumulation of cell cultures of T. cuspidata. The present study examined the influence of white light on the accumulation of taxol and baccatin III callus and cell suspension cultures of T. cuspidata. Results showed that taxol and baccatin III accumulations were approximately three times higher in dark-grown calli. Dark-grown suspensions also displayed three times more total volumetric taxol relative to light-grown suspensions; baccatin III amounts were similar in dark and light grown suspensions. Growth of suspensions was two to three-fold higher in darkness. Taxol accumulation peaked at stationary phase (d27 to d45) in dark-grown cells (14mg . kg(-1)edw). The peak of baccatin III accumulation (180 mg . kg(-1)edw) preceded the taxol peak (d18 to d27) and the content of this taxoid decreased as taxol increased. Baccatin III accumulation in the culture medium was similar in dark and light-grown suspensions (peaked at 6.2 mg . L(-1) on d9). Taxol accumulation in the medium was higher in darkness (0.3 mg . L(-1) on d9 and d27 to d36) than in light (0.08 mg . L(-1) on d9). Down-regulation of taxol steady-state amounts may be related to the effect of light on microsomal 3-hydroxy-3-methylglutaryl-coenzyme A reductase and mevalonate supply; the enzyme had significantly lower activity in light-grown cells.