Alpha-Tubulin and Small Subunit rRNA Phylogenies of Peritrichs Are Congruent and Do Not Support the Clustering of Mobilids and Sessilids (Ciliophora, Oligohymenophorea)
Alpha-Tubulin and Small Subunit rRNA Phylogenies of Peritrichs Are Congruent and Do Not Support the Clustering of Mobilids and Sessilids (Ciliophora, Oligohymenophorea)
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Peritrichs 的 α-微管蛋白和小亚基 rRNA 系统发育是一致的，不支持 Mobilids 和 Sessilids（纤毛纲、少膜纲）的聚类
DOI：
10.1111/j.1550-7408.2010.00472.x
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发表时间：
2010-05-01
影响因子：
2.2
通讯作者：
Feng, Weisong
中科院分区：
文献类型：
--
作者：
Gong, Yingchun;Xu, Kuidong;Feng, Weisong
周毛纤毛虫传统上被分为两个目，即周毛亚纲中的无柄纤毛虫目（Sessilida）和游动纤毛虫目（Mobilida）。然而，所有现有的小亚基（SSU）rRNA系统发育树显示，无柄和游动没有分支在一起。为了阐明这一分歧，我们通过评估SSU rRNA基因发生与α-微管蛋白基因发生一致性的可靠性来测试经典的周毛虫属是否是一个单系群。为此，我们获得了10个部分的α-微管蛋白序列从peritrichs和α-微管蛋白的核苷酸和氨基酸数据的基础上建立系统发育树。还构建了来自α-微管蛋白和SSU rRNA基因组合的系统发育树，并使用相似的物种采样与来自SSU rRNA基因的系统发育树进行比较。我们的研究结果表明，mobilids和sessilids始终分离在所有的树木，这加强了这样的想法，peritrichs不构成一个单系群。然而，在所有的α-微管蛋白基因树中，壶形虫和壶形虫没有聚在一起，这表明游动虫可能不是一个单系群。
Peritrich ciliates have been traditionally subdivided into two orders, Sessilida and Mobilida within the subclass Peritrichia. However, all the existing small subunit (SSU) rRNA phylogenetic trees showed that the sessilids and mobilids did not branch together. To shed some light on this disagreement, we tested whether or not the classic Peritrichia is a monophyletic group by assessing the reliability of the SSU rRNA phylogeny in terms of congruency with alpha-tubulin phylogeny. For this purpose, we obtained 10 partial alpha-tubulin sequences from peritrichs and built phylogenetic trees based on alpha-tubulin nucleotide and amino acid data. A phylogenetic tree from the alpha-tubulin and SSU rRNA genes in combination was also constructed and compared with that from the SSU rRNA gene using a similar species sampling. Our results show that the mobilids and sessilids are consistently separated in all trees, which reinforces the idea that the peritrichs do not constitute a monophyletic group. However, in all alpha-tubulin gene trees, the urceolariids and trichodiniids do not group together, suggested mobilids may not be a monophyletic group.