DIFFUSION-COEFFICIENTS OF GLUCOSE AND ETHANOL IN CELL-FREE AND CELL-OCCUPIED CALCIUM ALGINATE MEMBRANES
DIFFUSION-COEFFICIENTS OF GLUCOSE AND ETHANOL IN CELL-FREE AND CELL-OCCUPIED CALCIUM ALGINATE MEMBRANES
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DOI：
10.1002/bit.260280609
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发表时间：
1986-06-01
影响因子：
3.8
通讯作者：
STEPHANOPOULOS, G
中科院分区：
文献类型：
--
作者：
HANNOUN, BJM;STEPHANOPOULOS, G
在扩散池中测定了葡萄糖和乙醇在无细胞和有细胞海藻酸钙膜中的扩散系数。采用滞后时间分析法。扩散系数随着藻酸盐浓度的增加而降低，并且与2%藻酸盐膜在水中的扩散系数相当。葡萄糖和乙醇浓度对各自的扩散系数没有影响。在2和4%的海藻酸盐中的乙醇扩散率与葡萄糖扩散率的比率与两个分子在水中的流体动力学半径的反比密切相关，这表明在液体中扩散的流体动力学理论可适用于在稀海藻酸盐凝胶中的扩散。此外，存在20%的死酵母细胞对扩散率没有影响。所报道的数据可用于研究固定化细胞反应器中的反应和扩散以及固定化条件下的细胞生理学。
The diffusivities of glucose and ethanol in cell-free and cell-occupied membranes of calcium alginate were measured in a diffusion cell. The lag time analysis was used. Diffusivities decreased with increasing alginate concentration and were comparable with those in water for a 2% alginate membrane. Glucose and ethanol concentrations had no effect on the respective diffusion coefficients. The ratio of ethanol diffusivity to glucose diffusivity in 2 and 4% alginate agreed closely with the inverse ratio of the hydrodynamic radii for the two molecules in water, indicating that the hydrodynamic theory of diffusion in liquids may be applicable to diffusion in dilute alginate gels. Also, the presence of 20% dead yeast cells had no effect on the diffusivities. The data reported can be used to study reaction and diffusion in immobilized cell reactors and cell physiology under immobilized conditions.