CRUSTACEAN CARDIOACTIVE PEPTIDE-IMMUNOREACTIVE NEURONS IN THE HAWKMOTH MANDUCA-SEXTA AND CHANGES IN THEIR IMMUNOREACTIVITY DURING POSTEMBRYONIC DEVELOPMENT
CRUSTACEAN CARDIOACTIVE PEPTIDE-IMMUNOREACTIVE NEURONS IN THE HAWKMOTH MANDUCA-SEXTA AND CHANGES IN THEIR IMMUNOREACTIVITY DURING POSTEMBRYONIC DEVELOPMENT
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DOI：
10.1002/cne.903380410
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发表时间：
1993-12-22
影响因子：
2.5
通讯作者：
HILDEBRAND, JG
中科院分区：
文献类型：
--
作者：
DAVIS, NT;HOMBERG, U;HILDEBRAND, JG
用甲壳动物心脏活性多肽的抗血清，用间接免疫细胞化学方法，对鹰蛾胚胎后发育不同阶段的免疫反应神经元进行了定位。共鉴定出约90个免疫反应阳性神经元。其中许多细胞在孵化时呈免疫反应，并持续到成体阶段；其他细胞在胚胎后发育后期变得免疫反应。在成人发育过程中，瞬时免疫反应在食道下神经节和胸神经节的几个细胞中表达。在幼虫的前脑中发现了两组免疫反应神经元，但其中只有一组能持续到成虫阶段。成对的外侧中间神经元和神经分泌神经元在腹神经节内呈节段性重复，从一龄幼虫到成年期都存在；腹部中间神经元对侧投射到相邻神经节的树枝，有些上升到三脑树枝。腹部神经分泌细胞对应于一对据报道含有法氏囊的细胞，向后投射到神经血液释放器官。腹外斜肌背侧运动神经元在4龄幼虫时开始出现免疫反应。腹神经节成对的正中神经分泌细胞在第五龄幼虫时开始免疫反应。免疫反应阳性的正中和侧腹神经分泌细胞是一组已知含有心脏活性多肽的细胞的子集。食道下神经节的成对外侧神经分泌细胞在化蛹过程中呈免疫反应，并投射到成虫的心体和主动脉。这里发现的许多神经元与先前在蝗虫和粉虫中描述的甲壳类心脏活性多肽免疫反应细胞相似。(C)1993年Wiley-Liss，Inc.
An antiserum against crustacean cardioactive peptide was used, in indirect immunocytochemistry on whole-mounts and Vibratome sections, to map immunoreactive neurons at various stages of postembryonic development of the hawkmoth Manduca sexta. About 90 immunoreactive neurons were identified. Many of these cells are immunoreactive at hatching and persist into the adult stage; others become immunoreactive late in postembryonic development. During adult development, transient immunoreactivity is expressed in several cells in the subesophageal and thoracic ganglia. Two sets of immunoreactive neurons are found in the protocerebrum of larvae, but only one of these sets persists into the adult stage. Paired lateral interneurons and neurosecretory neurons are segmentally repeated in the abdominal ganglia and are present from the first larval stage to the adult; the abdominal interneurons project contralaterally to arborizations in adjacent ganglia, and some ascend to tritocerebral arborizations. The abdominal neurosecretory cells, which correspond to a pair of cells reported to contain bursicon, project posteriorly to neurohemal release organs. Motor neurons of dorsal external oblique abdominal muscles become immunoreactive in the fourth larval stage. Paired median neurosecretory cells of abdominal ganglia become immunoreactive during the fifth larval stage. The immunoreactive median and lateral abdominal neurosecretory cells are a subset of a group of cells known to contain cardioactive peptides. Paired lateral neurosecretory cells of the subesophageal ganglion become immunoreactive during pupation and project to the corpora cardiaca and aorta of the adult. Many of the neurons identified here are comparable to crustacean cardioactive peptide-immunoreactive cells described previously in locusts and the mealworm beetle. (C)1993 Wiley-Liss, Inc.