Posterior vitreous detachment induced by microplasmin
Posterior vitreous detachment induced by microplasmin
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DOI：
10.1167/iovs.03-0930
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发表时间：
2004-02-01
影响因子：
4.4
通讯作者：
Charteris, D
中科院分区：
文献类型：
--
作者：
Gandorfer, A;Rohleder, M;Charteris, D
目的.证明微纤溶酶在。诱导玻璃体后脱离（PVD），并对治疗后的人和猫视网膜进行评价。13只人供体眼睛注射62.5、125或188 μ g微纤溶酶。13只对侧眼接受平衡盐溶液。四只微纤溶酶治疗的眼睛接受了额外的玻璃体内气体注射。在37 ℃下孵育30分钟后，将所有球体置于4%多聚甲醛中。视网膜标本进行扫描（SEM）和透射（TEM）电子显微镜。五只猫的眼睛注射14.5或25 μ g的微纤溶酶。在1天、3天或3周后处死动物，并通过电子和共聚焦显微镜评价视网膜标本。在所有对照眼中，扫描电镜显示皮质玻璃体覆盖内界膜（ILM）。玻璃体内注射125或188 μ g微纤溶酶导致完全PVD。用62.5 μ g微纤溶酶处理后，SEM显示胶原纤维覆盖ILM。额外的气体注入没有改变PVD所需的剂量。在猫体内，25 μ g微纤溶酶在3天后导致完全PVD。3周后，两种剂量的微纤溶酶均出现完全PVD。视网膜及内界膜均保存良好。无论是在死后还是在体内，微纤溶酶诱导玻璃体皮质和ILM之间的剂量依赖性分裂，而没有视网膜的形态学改变。在猫的眼睛中，视网膜神经胶质细胞或神经元没有细胞反应。
PURPOSE. To demonstrate the efficacy of microplasmin in. inducing posterior vitreous detachment (PVD) and to evaluate the human and the feline retina after treatment.METHODS. Thirteen human donor eyes were injected with 62.5, 125, or 188 jig microplasmin. The 13 fellow eyes received balanced salt solution. Four of the microplasmin-treated eyes received an additional intravitreal gas injection. After incubation at 37degreesC for 30 minutes, all globes were placed in 4% paraformaldehyde. Retinal specimens were processed for scanning (SEM) and transmission (TEM) electron microscopy. Five feline eyes were injected with 14.5- or 25-mug microplasmin. Animals were killed after 1 day, 3 days, or 3 weeks, and retinal specimens were evaluated by electron and confocal microscopy.RESULTS. In all control eyes, SEM demonstrated the cortical vitreous covering the inner limiting membrane (ILM). Intravitreal injection of 125 or 188 mug microplasmin resulted in complete PVD. After treatment with 62.5 mug microplasmin, SEM revealed collagen fibrils covering the ILM. Additional gas injection did not change the dose necessary for PVD. In vivo in cats, 25 mug microplasmin resulted in complete PVD after 3 days. After 3 weeks, there was complete PVD with both doses of microplasmin. The retina and the ILM were well preserved in all eyes.CONCLUSIONS. Both after death and in vivo, microplasmin induces a dose-dependent cleavage between the vitreous cortex and the ILM without morphologic alterations of the retina. In the feline eye, there is no cellular response of retinal glial cells or neurons.