Anoxia or oxygen and glucose deprivation in SH-SY5Y cells: A step closer to the unraveling of neuroglobin and cytoglobin functions
Anoxia or oxygen and glucose deprivation in SH-SY5Y cells: A step closer to the unraveling of neuroglobin and cytoglobin functions
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DOI：
10.1016/j.gene.2007.03.022
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发表时间：
2007-08-15
期刊：
影响因子：
3.5
通讯作者：
Dewilde, Sylvia
中科院分区：
文献类型：
--
作者：
Fordel, Elke;Thijs, Liesbet;Dewilde, Sylvia
几项研究支持这一假设，即当细胞供氧至关重要时，脑红蛋白和细胞红蛋白对细胞死亡起保护作用。虽然潜在的分子机制是未知的，以前的报告表明，这种保护可以实现的事实，他们作为活性氧清除剂。在这项研究中，神经红蛋白和细胞红蛋白的表达在人神经母细胞瘤细胞系（SH-SY5Y）缺氧或氧和葡萄糖剥夺（OGD）的条件下进行了评估。细胞可在长时间缺氧条件下存活，且存活率无明显下降。缺糖后，它们变得缺氧敏感。OGD在16小时后诱导显著的细胞死亡，导致32小时后54%的细胞死亡。细胞坏死是OGD诱导细胞死亡的主要过程。复氧后，凋亡神经元变得更加丰富。实时定量PCR和蛋白质印迹显示，脑红蛋白和细胞红蛋白上调，前者在OGD和后者在缺氧条件下。在OGD条件下，用反义ODN转染抑制细胞珠蛋白表达后，细胞存活率显著降低。此外，神经红蛋白或细胞红蛋白过表达显著增强细胞存活。当脑红蛋白或细胞红蛋白的蛋白质表达增加或减少，H2O2水平被发现是较低或较高，分别。我们的结论是，脑红蛋白或细胞红蛋白作为活性氧清除剂在缺血条件下。(c)2007 Elsevier B.V.保留所有权利。
Several studies support the hypothesis that neuroglobin and cytoglobin play a protective role against cell death when cellular oxygen supply is critical. Although the underlying molecular mechanisms are unknown, previous reports suggest that this protection can be realised by the fact that they act as ROS scavengers. In this study, expression of neuroglobin and cytoglobin was evaluated in a human neuroblastoma cell line (SH-SY5Y) under conditions of anoxia or oxygen and glucose deprivation (OGD). The cells could survive prolonged anoxia without significant loss of viability. They became anoxia sensitive when deprived of glucose. OGD induced significant cell death after 16 h resulting in 54% dead cells after 32 h. Necrosis was the main process involved in OGD-induced cell death. After reoxygenation, apoptotic neurons became more abundant. Real-time quantitative PCR and Western blotting revealed that neuroglobin and cytoglobin were upregulated, the former under OGD and the latter under anoxic conditions. Under OGD, cell survival was significantly reduced after inhibiting cytoglobin expression by transfection with antisense ODN. Moreover, cell survival was significantly enhanced by neuroglobin or cytoglobin overexpression. When neuroglobin or cytoglobin protein expression increased or decreased, the H2O2 level was found to be lower or higher, respectively. We conclude that neuroglobin or cytoglobin act as ROS scavengers under ischemic conditions. (c) 2007 Elsevier B.V. All rights reserved.