Aspergillus fumigatus CYP51 sequence: potential basis for fluconazole resistance.
Aspergillus fumigatus CYP51 sequence: potential basis for fluconazole resistance.
复制标题
烟曲霉 CYP51 序列：氟康唑耐药性的潜在基础。
DOI：
10.1080/mmy.39.3.299.302
复制
发表时间：
2001
期刊：
影响因子：
2.9
通讯作者：
Katiyar,SK
中科院分区：
文献类型：
--
作者：
Edlind,TD;Henry,KW;Metera,KA;Katiyar,SK
我们克隆并测序了烟曲霉cyp51基因，该基因编码了唑类抗真菌药物的靶标细胞色素P450甾醇14α去甲基化酶。SinceA。烟曲霉病对广泛使用的氟康唑具有内在抗性，我们将其预测的CYP51序列与氟康唑敏感和耐药白色念珠菌的CYP51序列进行了比较。这一分析产生了关于a的基础的具体假设。fumigatusfluconazole阻力;特别是,一个。熏蒸菌残留Ile301对应于c。白念珠菌残基Thr315在抗性菌株中突变为Ala，并被认为与甾醇底物形成氢键。
We have cloned and sequenced theAspergillus fumigatusCYP51 gene which encodes the target of azole antifungal agents, namely cytochrome P450 sterol 14αdemethylase. SinceA. fumigatusis intrinsically resistant to the widely used azole fluconazole, we compared its predicted CYP51 sequence to the CYP51 sequences from fluconazole-susceptible and resistantCandida albicans. This analysis generated specific hypotheses regarding the basis forA. fumigatusfluconazole resistance; in particular,A. fumigatusresidue Ile301 corresponds toC. albicansresidue Thr315 which is mutated to Ala in resistant strains and is proposed to hydrogen bond with the sterol substrate.