Dual-Wavelength Ratiometric Electrochemiluminescence Immunosensor for Cardiac Troponin I Detection
Dual-Wavelength Ratiometric Electrochemiluminescence Immunosensor for Cardiac Troponin I Detection
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DOI：
10.1021/acs.analchem.8b04640
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发表时间：
2019-01-15
影响因子：
7.4
通讯作者：
Yang, Xiurong
中科院分区：
文献类型：
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作者：
Ye, Jing;Zhu, Liping;Yang, Xiurong
比率型电化学发光（ECL）因其能消除环境干扰、实现精确测量而受到生物分析领域的关注。本文设计了一种用于检测心肌肌钙蛋白I（cTnI）的双波长比率电化学发光生物传感器，其中（4，4 '-二羧酸-2，2'-联吡啶）钌（II）（Ru（dcbpy）（3）（2+））和Au纳米颗粒负载的氧化石墨烯/聚乙烯亚胺（GPRu-Au）纳米材料作为受体，Au纳米颗粒修饰的石墨相氮化碳纳米片复合物（Au-CNN）作为供体。Au-CNN显示出中心在455 nm处的高且稳定的ECL信号，这与GPRu Au的吸附良好匹配;由此，设计了高效的电致发光共振能量转移（ECL-RET）传感平台。纳米金通过Au-N键促进抗体在纳米材料上的固定。氧化石墨烯/聚乙烯亚胺的高表面积允许负载大量Ru（dcbpy）（3）（2+），极大地放大ECL信号。该传感平台对cTnI具有出色的分析性能，检测限为3.94 fg/mL（S/N = 3）。该比率型ECL生物传感器的高可靠性、选择性和灵敏度为生物分析提供了一个通用的传感平台。
Ratiometric electrochemiluminescence (ECL) has attracted special focus in the biological analysis field, because it could eliminate the environmental interference and allow for precise measurement. Herein, a dual-wavelength ratiometric ECL biosensor was designed for the detection of cardiac troponin I (cTnI), where (4,4'-dicarboxylic acid-2,2'-bipyridyl) ruthenium(II) (Ru(dcbpy)(3)(2+)) and Au nano particle-loaded graphene oxide/polyethylenimine (GPRu-Au) nanomaterial acts as an acceptor, and Au nanoparticle-modified graphitic phase carbon nitride nanosheet composite (Au-CNN) acts as donor. Au-CNN shows a high and steady ECL signal centered at 455 nm, which is well-matched with the adsorption of GPRu Au; thereby, a highly efficient electrochemiluminescent resonance energy transfer (ECL-RET) sensing platform is designed. AuNPs facilitate the immobilization of antibody on the nanomaterials through a Au-N bond. The high surface area of graphene oxide/polyethylenimine allows a large number of Ru(dcbpy)(3)(2+) to be loaded, immensely amplifying the ECL signal. This sensing platform exhibits outstanding analytical performance toward cTnI with a detection limit of 3.94 fg/mL (S/N = 3). The high reliability, selectivity, and sensitivity of this ratiometric ECL biosensor provides a versatile sensing platform for the bioanalysis.