Development and integration of an immunoaffinity monolithic disk for the on-line solid-phase extraction and HPLC determination with fluorescence detection of aflatoxin B1 in aqueous solutions
Development and integration of an immunoaffinity monolithic disk for the on-line solid-phase extraction and HPLC determination with fluorescence detection of aflatoxin B1 in aqueous solutions
复制标题
DOI：
10.1016/j.jpba.2007.01.030
复制
发表时间：
2007-06-28
影响因子：
3.4
通讯作者：
Caccialanza, G.
中科院分区：
文献类型：
--
作者：
Calleri, E.;Marrubini, G.;Caccialanza, G.
本文报道了一种抗黄曲霉毒素B_1（anti-AFB_1）免疫亲和整体盘的研制和鉴定。将多克隆抗-AFB 1批量共价固定在环氧活化的整体对流相互作用介质（CIM）盘（12 mm x 3 mm i.d.）通过聚合物表面的环氧基团的一步反应。0.96将20 mg抗体固定化，并通过前沿分析测定CIM盘的结合容量。CIM盘通过切换阀连接到反相柱，即Chromolith Performance RP-18 e。建立了水溶液中黄曲霉毒素B I的全自动高效液相色谱荧光检测法。与集成系统的总分析时间为46分钟，黄曲霉毒素B1的保留时间约为29分钟。免疫亲和盘的结合能力进行了评价的线性，精密度和准确度的提取程序。免疫亲和支持物在重复运行后是稳定的。(c)2007 Elsevier B. V.保留所有权利。
The development and characterization of an anti-aflatoxin B1 (anti-AFB1) immunoaffinity monolithic disk is reported. Polyclonal anti-AFB1 was covalently immobilized in batch on an epoxy-activated monolithic Convective Interaction Media (CIM) disk (12 mm x 3 mm i.d.) by a one-step reaction via epoxy groups of the polymer surface. 0.96 mg of antibody were immobilized and the binding capacity of the CIM disk was determined by frontal analysis. The CIM disk was coupled through a switching valve to a reversed-phase column, namely Chromolith Performance RP-18e. A fully automated HPLC method with fluorescence detection for the determination of aflatoxin B I in aqueous solution was developed. The total analysis time with the integrated system is 46 min and the retention time of AFB1 is approximately 29 min. The binding capacity of the immumoaffinity disk was evaluated in terms of linearity, precision and accuracy of the extraction procedure. The immunoaffinity support was stable after repeated runs. (c) 2007 Elsevier B.V. All rights reserved.