Functional analysis of OsHSBP1 and OsHSBP2 revealed their involvement in the heat shock response in rice (Oryza sativa L.)
Functional analysis of OsHSBP1 and OsHSBP2 revealed their involvement in the heat shock response in rice (Oryza sativa L.)
复制标题
OsHSBP1 和 OsHSBP2 的功能分析揭示了它们参与水稻 (Oryza sativa L.) 的热休克反应
DOI：
10.1093/jxb/ers245
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发表时间：
2012-10-01
影响因子：
6.9
通讯作者：
Zhang, Hongsheng
中科院分区：
文献类型：
--
作者：
Rana, Rashid Mehmood;Dong, Shinan;Zhang, Hongsheng
热休克反应（HSR）通过激活热休克因子（HSF）诱导热休克蛋白（HSP）的产生。据报道，HSF 结合蛋白 (HSBP) 通过与其三聚体结合来调节 HSF 的功能，从而调节 HSR。该报告描述了 OsHSBP1 和 OsHSBP2 在调控水稻 HSR 和种子发育中的作用。在正常生长条件下，这两种基因在所有组织中普遍表达，而在热激处理后的恢复过程中，它们的表达水平显着增加。亚细胞定位揭示了洋葱表皮细胞中 OsHSBP1 和 OsHSBP2 的细胞质核定位。酵母双杂交测定描绘了两种基因的自结合能力。这两个基因对于种子发育也很重要，因为它们的敲低品系与严重的种子败育有关。耐热性测定表明，OsHSBP1和OsHSBP2是HSR的负调节因子，参与获得性耐热性，但不参与基础耐热性，因为它们的过表达转基因株系在亚致死温度下预热，在热激处理后显示出显着降低的幼苗存活率。此外，与野生型相比，热应激后OsHSBP1和OsHSBP2敲低转基因幼苗的抗氧化活性以及过氧化氢酶和过氧化物酶基因表达显着增加。热特异性 HSP 的表达在这两个基因的敲除系中也显着增加，但以特定的方式增加，表明 HSBP 基因参与不同的途径。总的来说，本研究揭示了 OsHSBP1 和 OsHSBP2 在调控水稻 HSR 和种子发育中的作用。
The heat shock response (HSR) induces the production of heat shock proteins (HSPs) through the activation of heat shock factors (HSF). HSF binding protein (HSBP) is reported to modulate the function of HSF by binding to their trimer and hence to regulate HSR. This report describes the role of OsHSBP1 and OsHSBP2 in the regulation of the HSR and seed development of rice. Both genes expressed ubiquitously in all tissues under normal growth conditions while their expression levels were significantly increased during recovery after heat shock treatment. Subcellular localization revealed the cytosolnuclear localization of both OsHSBP1 and OsHSBP2 in onion epidermal cells. The yeast two-hybrid assay depicted the self-binding ability of both genes. Both genes were also important for seed development, as their knock-down lines were associated with significant seed abortion. The thermotolerance assay revealed that OsHSBP1 and OsHSBP2 are negative regulators of HSR and involved in acquired thermotolerance but not in basal thermotolerance since their over-expression transgenic lines pre-heated at sublethal temperature, showed significantly decreased seedling survival after heat shock treatment. Furthermore, antioxidant activity and gene expression of catalase and peroxidase was significantly increased in knock-down transgenic seedlings of OsHSBP1 and OsHSBP2 after heat stress compared with the wild type. The expression of heat specific HSPs was also increased significantly in knockdown line of both genes but in a specific manner, suggesting the involvement of HSBP genes in different pathways. Overall, the present study reveals the role of OsHSBP1 and OsHSBP2 in the regulation of the HSR and seed development of rice.