T-CELL RECEPTOR BETA-CHAIN EXPRESSION - DEPENDENCE ON RELATIVELY FEW VARIABLE REGION GENES
T-CELL RECEPTOR BETA-CHAIN EXPRESSION - DEPENDENCE ON RELATIVELY FEW VARIABLE REGION GENES
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DOI：
10.1126/science.3875151
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发表时间：
1985-01-01
期刊：
影响因子：
56.9
通讯作者：
LOH, DY
中科院分区：
文献类型：
--
作者：
BEHLKE, MA;SPINELLA, DG;LOH, DY
15个独立分离的互补DNA克隆包含T细胞受体（TCR） v . β。对11种不同的v . β基因进行了测序。基因。与已知序列相比，14种不同的v . β。从25个互补DNA中可以确定基因；因此，11个克隆涉及重复使用先前鉴定的V.beta。种系v . β表达数的最大似然估计。基因数计算为18,95%置信区间为30个基因。Southern blot分析表明，这些基因大多属于单元素亚家族，株系间多态性非常有限。TCR。beta。-链多样性似乎是由有限的v - β产生的。变异主要是由可变多样性连接（V-D-J）连接位点的广泛变异引起的，没有证据表明体细胞超突变参与其中。
Fifteen independently isolated complementary DNA clones that contain T cell receptor (TCR) V.beta. genes were sequenced and represented 11 different V.beta. genes. When compared with known sequences, 14 different V.beta. genes could be defined from a total of 25 complementary DNA; 11 clones therefore involved repeated usage of previously identified V.beta.. A maximum likelihood estimate of the number of expressed germline V.beta. genes was calculated as 18 with an upper 95% confidence bond of 30 genes. Southern blot analysis has shown that most of these genes belong to single element subfamilies which show very limited interstrain polymorphism. The TCR .beta.-chain diversity appears to be generated from a limited V.beta. gene pool primarily by extensive variability at the variable-diversity-joining (V-D-J) junctional site, with no evidence for the involvement of somatic hypermutation.