Isolation and characterization of a cinnamoyl-CoA reductase gene from perennial ryegrass (Lolium perenne)
Isolation and characterization of a cinnamoyl-CoA reductase gene from perennial ryegrass (Lolium perenne)
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DOI：
10.1078/0176-1617-00719
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发表时间：
2002-04-01
影响因子：
4.3
通讯作者：
Spangenberg, G
中科院分区：
文献类型：
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作者：
McInnes, R;Lidgett, A;Spangenberg, G
肉桂酰辅酶A还原酶(Ccr，EC 1.2.1.44)催化木质素生物合成途径中单醇专一性分支的入口点将羟基肉桂酰辅酶A硫酯转化为肉桂醛。克隆了多年生黑麦草CCR基因(LpCCR1)，并进行了全序列测定。LpCCR1含有5个大小与其他CCR相似的外显子和4个保守位置的内含子。5‘非翻译区含有木质素生物合成基因共有的基序。在多年生黑麦草中，LpCCR1以单拷贝基因的形式存在，并定位于LG7。LpCCR1是结构性表达的，受机械伤害的刺激，茎中的转录丰度随着成熟度的增加而增加。
Cinnamoyl-CoA reductase (CCR, EC 1.2.1.44) catalyses the conversion of hydroxycinnamoyl-CoA thioesters to cinnamaldehydes at the entry point to the monolignol-specific branch of the lignin biosynthetic pathway. A gene encoding CCR in perennial ryegrass (LpCCR1) was isolated and fully sequenced. LpCCR1 contains five exons of similar sizes to other CCRs and four introns in conserved positions. The 5' untranslated region contains motifs common to lignin biosynthetic genes. LpCCR1 is present as a single copy gene and maps to LG7 in perennial ryegrass. LpCCR1 is constitutively expressed, is stimulated by mechanical wounding, and transcript abundance in stems increases with maturity.