Change in the expressed gene patterns of the wing disc during the metamorphosis of Bombyx mori
Change in the expressed gene patterns of the wing disc during the metamorphosis of Bombyx mori
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DOI：
10.1016/j.gene.2004.08.013
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发表时间：
2004-12-08
期刊：
影响因子：
3.5
通讯作者：
Mita, K
中科院分区：
文献类型：
--
作者：
Kawasaki, H;Ote, M;Mita, K
本研究旨在阐明昆虫成虫盘在变态过程中发生了什么。为了在分子水平上了解变态，研究了变态过程中成虫盘中表达谱的变化。为此，我们构建了家蚕四个不同发育阶段翅盘的cDNA文库，并对每个文库中随机收集的约1000个cDNA进行了测序，构建了表达序列标签（EST）数据库。比较了这四个时期的形态变化和EST表达谱。利用基因芯片技术分析了各基因在各阶段的表达量，以确定EST中所鉴定的基因的表达是否受20-羟基蜕皮激素（20-hydroxyecdysone，20 E）的诱导，并具有阶段特异性。通过比较每个EST克隆在四个不同时期的表达谱，发现每个EST克隆在不同时期的表达谱不同。这些剖面图反映了黄鳍文昌鱼翅盘在变态过程中的形态变化。结果表明，V4期为细胞增殖期，WO期为细胞增殖分化期，W2期为细胞形态发生变化期，W3期为角质层分泌期。基因芯片分析表明，其应用于20 E诱导的基因在翅盘的有效性。B的翼盘。Mori是一个非常适合于理解形态变化和mRNA分布之间关系的系统。由爱思唯尔公司出版
The present study was conducted to clarify what occurs during the metamorphosis of the imaginal disc in insects. To understand the metamorphosis on a molecular level, the changes in expression profiles in the imaginal disc during metamorphosis were investigated. For this purpose, we constructed cDNA libraries from four different stages of wing discs of Bombyx mori, sequenced about 1000 cDNAs randomly collected from each library, and constructed a database of expressed sequence tags (EST). The morphological changes and expression profiles from EST were compared during those four stages. Microarray analysis was applied to quantify the expression of each gene in each stage in order to confirm whether the expression of the genes identified from EST was induced by 20-hydroxyecdysone (20E) in a stage-specific manner.Wing discs showed dynamic morphogenesis in 4-5 days during the preparatory stage of metamorphosis, which was under the control of an ecdysteroid. Different expressed profiles were observed in each of the four different stages by comparison of each EST clone. These profiles reflected the morphological changes of the Bombyx wing disc during metamorphosis. The results of expression profiles from the four stages suggested that the V4 stage was cell proliferating; WO, proliferating and the beginning of differentiation; W2, morphologically changing; W3, cuticle secreting. Microarray analysis showed the effectiveness of its application on 20E induction of genes in wing discs. The wing disc of B. mori is an exceptionally suitable system for understanding the relationship between morphological changes and the distribution of mRNA. Published by Elsevier B.V.