The transcription factor TaLAX1 interacts with Q to antagonistically regulate grain threshability and spike morphogenesis in bread wheat
The transcription factor TaLAX1 interacts with Q to antagonistically regulate grain threshability and spike morphogenesis in bread wheat
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DOI：
10.1111/nph.17235
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发表时间：
2021-02-22
期刊：
影响因子：
9.4
通讯作者：
Sun, Jiaqiang
中科院分区：
文献类型：
--
作者：
He, Guanhua;Zhang, Yunwei;Sun, Jiaqiang
驯化基因 Q 在很大程度上促进了小麦的广泛种植，因为它赋予了多种驯化性状。然而，Q 如何调节这些驯化性状的潜在分子机制仍不清楚。在这项研究中，我们通过酵母双杂交测定鉴定了一种 Q 相互作用蛋白 TaLAX1，一种基本的螺旋-环-螺旋转录因子。利用生化和遗传学方法，我们探索了 TaLAX1 在调节小麦驯化性状中的作用。TaLAX1 的过度表达会产生令人想起 q 等位基因的表型；功能缺失的 Talax1 突变会产生紧凑的尖峰，与 Q 过度表达的小麦品系非常相似。 TaLAX1和Q这两个转录因子相互干扰对方的活性，拮抗调节木质素生物合成相关基因TaKNAT7-4D的表达。更有趣的是，TaLAX1启动子中发生的自然变异（InDel，+/- TATA）与面包小麦D亚基因组与其祖先节节山羊草品种T093之间的启动子活性差异有关。本研究揭示转录因子TaLAX1与Q物理相互作用，拮抗调节小麦驯化性状，并产生自然变异（InDel，+/- TATA）。 TATA）与 TaLAX1 启动子活性的多样化相关。
The domestication gene Q is largely responsible for the widespread cultivation of wheat because it confers multiple domestication traits. However, the underlying molecular mechanisms of how Q regulates these domestication traits remain unclear.In this study, we identify a Q-interacting protein TaLAX1, a basic helix-loop-helix transcription factor, through yeast two-hybrid assays. Using biochemical and genetic approaches, we explore the roles of TaLAX1 in regulating wheat domestication traits.Overexpression of TaLAX1 produces phenotypes, reminiscent of the q allele; loss-of-function Talax1 mutations confer compact spikes, largely similar to the Q-overexpression wheat lines. The two transcription factors TaLAX1 and Q disturb each other's activity to antagonistically regulate the expression of the lignin biosynthesis-related gene TaKNAT7-4D. More interestingly, a natural variation (InDel, +/- TATA), which occurs in the promoter of TaLAX1, is associated with the promoter activity difference between the D subgenome of bread wheat and its ancestor Aegilops tauschii accession T093.This study reveals that the transcription factor TaLAX1 physically interacts with Q to antagonistically regulate wheat domestication traits and a natural variation (InDel, +/- TATA) is associated with the diversification of TaLAX1 promoter activity.