A novel video-tracking system to quantify the behaviour of nocturnal mosquitoes attacking human hosts in the field.
A novel video-tracking system to quantify the behaviour of nocturnal mosquitoes attacking human hosts in the field.
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DOI：
10.1098/rsif.2015.0974
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发表时间：
2016-04
期刊：
影响因子：
--
通讯作者：
Towers DP
中科院分区：
文献类型：
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作者：
Angarita-Jaimes NC;Parker JE;Abe M;Mashauri F;Martine J;Towers CE;McCall PJ;Towers DP
许多疟疾和其他传染病的病媒成年后大部分时间都在人类家中度过，这是它们吸血和休息的环境，也是控制最成功的地方。然而，对家庭周围蚊子行为的了解有限，特别是蚊子如何找到和攻击人类宿主或杀虫剂如何影响行为。这部分是因为在疾病流行国家的自然栖息地（传统住所）跟踪蚊子的技术从未出现。我们描述了一种传感装置，可以在实验室和非洲农村观察和记录夜间蚊子攻击人类的情况，无论是否使用蚊帐。该设备满足了在2.0 × 1.2 × 2.0米体积内使用最小辐照度时的亚毫米分辨率要求。数据处理策略，以提取个体蚊子的轨迹和算法来描述主机/净相互作用过程中的行为。英国实验室和坦桑尼亚现场测试的结果表明，致倦库蚊活动较高，并集中在蚊帐屋顶时，人类宿主存在，在殖民地和野生种群。两个C.致倦按蚊和冈比亚按蚊表现出相似的行为模式，平均飞行速度变化小于10%。该系统为媒介生物学和许多其他领域的研究提供了相当大的潜力。
Many vectors of malaria and other infections spend most of their adult life within human homes, the environment where they bloodfeed and rest, and where control has been most successful. Yet, knowledge of peri-domestic mosquito behaviour is limited, particularly how mosquitoes find and attack human hosts or how insecticides impact on behaviour. This is partly because technology for tracking mosquitoes in their natural habitats, traditional dwellings in disease-endemic countries, has never been available. We describe a sensing device that enables observation and recording of nocturnal mosquitoes attacking humans with or without a bed net, in the laboratory and in rural Africa. The device addresses requirements for sub-millimetre resolution over a 2.0 × 1.2 × 2.0 m volume while using minimum irradiance. Data processing strategies to extract individual mosquito trajectories and algorithms to describe behaviour during host/net interactions are introduced. Results from UK laboratory and Tanzanian field tests showed that Culex quinquefasciatus activity was higher and focused on the bed net roof when a human host was present, in colonized and wild populations. Both C. quinquefasciatus and Anopheles gambiae exhibited similar behavioural modes, with average flight velocities varying by less than 10%. The system offers considerable potential for investigations in vector biology and many other fields.