Down-regulation of Homer1b/c expression protects cultured neurons after traumatic injury.
Down-regulation of Homer1b/c expression protects cultured neurons after traumatic injury.
复制标题
DOI：
10.3969/j.issn.1673-5374.2012.028.002
复制
发表时间：
2012-10-05
影响因子：
6.1
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Huang W;Liu X;Fei Z;Zhang Y;Yang J
代谢型谷氨酸受体1a的激活对创伤性脑损伤的影响。组成型表达的蛋白Homer 1b/c参与传递和锚定神经元中的代谢型谷氨酸受体。在这里，我们的目的是验证是否通过RNA干扰下调Homer 1b/c可以保护培养的大鼠皮层神经元免受创伤性损伤。我们发现，36小时后转染Homer 1b/c小干扰RNA，代谢型谷氨酸受体1a只存在于神经元的细胞质中，但不是在树突。钙荧光强度也明显降低。此外，乳酸脱氢酶浓度显着降低Homer 1b/c小干扰RNA转染细胞相比，在未转染和对照小干扰RNA转染细胞创伤性神经元损伤后24小时。我们的研究结果表明，下调Homer 1b/c可以减少代谢型谷氨酸受体1a从胞体到树突的转移，减轻钙超载，保护神经元免受创伤性损伤。
Activation of metabotropic glutamate receptor 1a aggravates traumatic brain injury. The constitutively expressed protein Homer1b/c participates in delivering and anchoring metabotropic glutamate receptors in neurons. Here, we aimed to verify whether down-regulation of Homer1b/c by RNA interference could protect cultured rat cortical neurons from traumatic injury. We showed that 36 hours after transfection of Homer1b/c small interfering RNA, metabotropic glutamate receptor 1a was present only in the neuronal cytoplasm, but not in the dendrites. Calcium fluorescence intensity was also decreased significantly. Moreover, lactate dehydrogenase concentration was significantly decreased in Homer1b/c small interfering RNA-transfected cells compared with that in untransfected and control small interfering RNA-transfected cells 24 hours after traumatic neuronal injury. Our findings indicate that down-regulation of Homer1b/c could reduce metabotropic glutamate receptor 1a transfer from the cell body to the dendrite, relieve calcium overload, and protect neurons from traumatic injury.
登录
查看更多内容
DOI：
10.1007/s00249-009-0494-1
发表时间：
2009-12-01
影响因子：
2
作者：
Pouliquin, Pierre;Dulhunty, Angela Fay
通讯作者：
Dulhunty, Angela Fay
DOI：
10.1111/j.1748-1716.2011.02319.x
发表时间：
2012-02
期刊：
Acta physiologica (Oxford, England)
影响因子：
--
作者：
Yuan JP;Lee KP;Hong JH;Muallem S
通讯作者：
Muallem S
影响因子：
64.8
作者：
Brakeman, PR;Lanahan, AA;Worley, PF
通讯作者：
Worley, PF
影响因子：
5.3
作者：
Faden, AI;O'Leary, DM;Movsesyan, VA
通讯作者：
Movsesyan, VA
影响因子：
5.3
作者：
Ronesi, Jennifer A.;Huber, Kimberly M.
通讯作者：
Huber, Kimberly M.