Nanomechanotransduction and interphase nuclear organization influence on genomic control
Nanomechanotransduction and interphase nuclear organization influence on genomic control
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DOI：
10.1002/jcb.21354
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发表时间：
2007-12-01
影响因子：
4
通讯作者：
Curtis, Adam S. G.
中科院分区：
文献类型：
--
作者：
Dalby, Matthew J.;Gadegaard, Nikolaj;Curtis, Adam S. G.
细胞通过机械调节或改变间期细胞核的形态和组织来改变其基因组调节的能力是细胞生物学中的关键问题。在这里，两种纳米形貌被用作改变细胞形态的模型表面，以研究成纤维细胞细胞核内的空间基因组变化。最初，对染色体对的着丝粒进行标记，并计算在不同底物上的平均距离。此外，使用Affymetrix全基因组基因芯片对基因组变化进行排序，以响应地形，并绘制这些变化在染色体上发生的位置。可以看出，随着细胞扩散的改变，染色体上的位置也发生了变化，基因调控也被观察到。我们假设，随着细胞和细胞核形态的变化，这可能会通过打开或关闭转录因子的染色体区域来改变转录的可能性。
The ability of cells to alter their genomic regulation in response to mechanical conditioning or through changes in morphology and the organization of the interphase nuclei are key questions in cell biology. Here, two nanotopographies have been used as a model surfaces to change cell morphology in order to investigate spatial genomic changes within the nuclei of fibroblasts. Initially, centromeres for chromosome pairs were labeled and the average distance on different substrates calculated. Further to this, Affymetrix whole genome GeneChips were used to rank genomic changes in response to topography and plot the whereabouts on the chromosomes these changes were occurring. It was seen that as cell spreading was changed, so were the positions along the chromosomes that gene regulations were being observed. We hypothesize that as changes in cell and thus nuclear morphology Occur, that this may alter the probability of transcription through opening or closing areas of the chromosomes to transcription factors.