Expression of the stem cell marker nestin in peripheral blood of patients with melanoma
Expression of the stem cell marker nestin in peripheral blood of patients with melanoma
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DOI：
10.1111/j.1365-2133.2010.09779.x
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发表时间：
2010-07-01
影响因子：
10.3
通讯作者：
Keilholz, U.
中科院分区：
文献类型：
--
作者：
Fusi, A.;Ochsenreither, S.;Keilholz, U.
研究背景对循环中黑色素瘤细胞的标志物一直很感兴趣.巢蛋白是一种神经上皮中间丝蛋白，被发现在黑色素瘤和各种癌症干细胞中表达。ObjectivesWe调查了nestin在黑色素瘤患者外周血中的表达。MethodsWe通过流式细胞术和定量逆转录聚合酶链反应分析了nestin在组织（n = 23）和血液样本（n = 102）中的表达。来自美国癌症联合委员会III-IV期黑色素瘤患者。结果流式细胞术检测健康志愿者外周血中未发现巢蛋白表达细胞。然而，在黑色素瘤患者中，巢蛋白表达于通过免疫磁性分选从外周血富集的黑色素瘤细胞的比例中。在黑色素瘤组织样品中，在编码巢蛋白和酪氨酸酶（P = 0中心点001）和黑色素A（P = 0中心点002）的mRNA之间发现显著相关性，而在血液中，仅观察到酪氨酸酶（P = 0中心点015）的显著相关性，而黑色素A（P = 0中心点53）没有显著相关性。巢蛋白的表达是较高的IV期患者相比，III/IV期没有疾病的证据，在高肿瘤负荷的患者，并呈正相关的酪氨酸酶和melan-A.ConclusionsNestin的表达被发现是一个额外的标志物循环黑色素瘤细胞的利益。前瞻性研究应与已用于黑色素瘤细胞检测的标记物相比，研究其潜在的附加信息价值。
P>BackgroundThere is continued interest in markers indicative of circulating melanoma cells. Nestin is a neuroepithelial intermediate filament protein that was found to be expressed in melanoma and in various cancer stem cells.ObjectivesWe investigated expression of nestin in peripheral blood of patients with melanoma.MethodsWe analysed nestin expression by flow cytometry and by quantitative reverse transcription-polymerase chain reaction both in tissues (n = 23) and in blood samples (n = 102) from patients with American Joint Committee on Cancer stage III-IV melanoma. Forty-six negative controls were also added.ResultsFlow cytometry did not reveal nestin-expressing cells in peripheral blood of healthy volunteers. In patients with melanoma, however, nestin protein was expressed in a proportion of melanoma cells enriched from peripheral blood by immunomagnetic sorting. In melanoma tissue samples a significant correlation was found between mRNAs coding for nestin and tyrosinase (P = 0 center dot 001) and melan-A (P = 0 center dot 002), whereas in blood a significant correlation was observed only for tyrosinase (P = 0 center dot 015), but not for melan-A (P = 0 center dot 53). Nestin expression was higher in stage IV patients compared with stage III/IV with no evidence of disease, in patients with high tumour burden, and was positively correlated to expression of tyrosinase and melan-A.ConclusionsNestin was found to be an additional marker of interest for circulating melanoma cells. Prospective studies should investigate its potential added informative value in comparison with markers already in use for melanoma cell detection.