Colorimetric detection of cancer biomarker based on pH induced color change
Colorimetric detection of cancer biomarker based on pH induced color change
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DOI：
10.1016/j.snb.2011.12.015
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发表时间：
2012-05-20
影响因子：
8.4
通讯作者：
Qian, Zhiyong
中科院分区：
文献类型：
--
作者：
Chu, Binyang;Qi, Tingting;Qian, Zhiyong
报道了一种基于 pH 诱导的颜色变化的癌症生物标志物前列腺特异性抗原 (PSA) 的简单、容易的比色免疫测定法。制备谷胱甘肽功能化金纳米粒子 (Glu-AuNPs) 并用作免疫测定的标记，同时选择多巴胺锚定的 Fe3O4 (DA-Fe3O4) 来固定抗 PSA 抗体 (Ab(1))。 AuNPs 和 Fe3O4 在 PSA 存在下进一步组装成免疫复合物。用磁铁分离免疫复合物后，剩下的AuNPs与甲基红溶液混合并显示颜色。不同浓度的PSA对应的不同颜色可以用肉眼直接检测到。这种新颖的免疫分析对于检测其他癌症生物标志物具有巨大的潜力。 (C) 2011 Elsevier B.V. 保留所有权利。
A simple, easy, colorimetric immunoassay for cancer biomarker prostate specific antigen (PSA) is reported based on pH induced color change. Glutathione functionalized gold nanoparticles (Glu-AuNPs) were prepared and used as label for the immunoassay, while dopamine anchored Fe3O4 (DA-Fe3O4) was selected to immobilize anti-PSA antibody (Ab(1)). AuNPs and Fe3O4 were further assembled into immuno-complex in the presence of PSA. After the separation of the immuno-complex using magnet, the left AuNPs was mixed with methyl red solution and displaying colors. Different colors corresponding to different concentrations of PSA can be directly detected with eyes. This novel immunoassay has great potential to the detection of other cancer biomarkers. (C) 2011 Elsevier B.V. All rights reserved.