Development of an MRI-visible nonviral vector for siRNA delivery targeting gastric cancer
Development of an MRI-visible nonviral vector for siRNA delivery targeting gastric cancer
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开发用于靶向胃癌的 siRNA 递送的 MRI 可见非病毒载体
DOI：
10.2147/ijn.s24083
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发表时间：
2012-01-01
影响因子：
8
通讯作者：
Shuai, Xintao
中科院分区：
文献类型：
--
作者：
Chen, Yinting;Wang, Weiwei;Shuai, Xintao
一种抗体导向的非病毒载体，聚乙二醇接枝的聚乙烯亚胺功能与超顺磁性氧化铁纳米粒子和胃癌相关的CD 44 v6单链可变区片段（scFv（CD 44 v6），-PEG-g-PEI-SPION），被构建为胃癌靶向和磁共振成像（MRI）可见的小干扰RNA（siRNA）的纳米载体。进行PEG-g-PEI-SPION和scFv（CD 44 v6）-PEG-g-PEI-SPION的生物物理表征，包括siRNA缩合能力、细胞活力和转染效率。靶向和非靶向纳米载体都能有效地在体外转移siRNA。通过荧光成像和免疫荧光染色分析与siRNA复合的纳米颗粒的细胞摄取和分布。此外，通过MRI和组织学分析在体内验证了胃癌靶向效果。这些结果表明，scFv（CD 44 v6）-PEG-g-PEI-SPION是一种很有前途的非病毒载体，可用于胃癌的基因治疗和诊断。
An antibody-directed nonviral vector, polyethylene glycol-grafted polyethylenimine functionalized with superparamagnetic iron oxide nanoparticles and a gastric cancer-associated CD44v6 single-chain variable fragment (scFv(CD44v6),-PEG-g-PEI-SPION), was constructed as a gastric cancer-targeting and magnetic resonance imaging (MRI)-visible nanocarrier for small interfering RNA ( siRNA) delivery. Biophysical characterization of PEG-g-PEI-SPION and scFv(CD44v6)-PEG-g-PEI-SPION was carried out, including siRNA condensation capacity, cell viability, and transfection efficiency. Both the targeting and nontargeting nanocarriers were effective for transferring siRNA in vitro. The cellular uptake and distribution of nanoparticles complexed with siRNA was analyzed by fluorescence imaging and immunofluorescent staining. Moreover, the gastric cancer-targeting effect was verified in vivo by MRI and histology analysis. These results indicate that scFv(CD44v6)-PEG-g-PEI-SPION is a promising nonviral vector for gastric cancer gene therapy and diagnosis.