Ischemia-Reperfusion Injury in Rat Skeletal Muscle Assessed with T2-Weighted and Dynamic Contrast-Enhanced MRI
Ischemia-Reperfusion Injury in Rat Skeletal Muscle Assessed with T2-Weighted and Dynamic Contrast-Enhanced MRI
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DOI：
10.1002/mrm.22801
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发表时间：
2011-08-01
影响因子：
3.3
通讯作者：
Strijkers, G. J.
中科院分区：
文献类型：
--
作者：
Loerakker, S.;Oomens, C. W. J.;Strijkers, G. J.
压力性溃疡是由于机械负荷导致软组织破裂的局部区域。易受影响的个体需要接受压力缓解策略，以防止长时间的负荷。因此，缺血再灌注损伤可能在压疮的病因学中起重要作用。为了研究缺血后灌注与骨骼肌完整性变化之间的相互关系，对棕色挪威大鼠的后肢进行 4 小时缺血，然后再灌注 2 小时。使用动态对比增强 MRI 检查灌注，并使用 T-2 加权 MRI 监测骨骼肌完整性的变化。动态对比增强 MRI 数据显示后肢缺血后分布不均匀，包括对比增强增强的区域（后肢的 14-76%）和无复流的区域（5-77%）。对于 T-2，在 4 小时缺血期间（从 34 毫秒到 41 毫秒）观察到整条腿逐渐增加。在再灌注阶段，观察到 T-2 的不均匀分布。对比度增强增加的区域与 T-2 向缺血前水平的减少（至 38 毫秒）相关，而无复流区域则表现出 T-2 进一步增加（至 42 毫秒）。这些结果表明，长时间缺血后的再灌注可能不完全，从而使缺血状态持续并加重组织损伤。 Magn Reson Med 66:528-537, 2011。(C) 2011 Wiley-Liss, Inc.
Pressure ulcers are localized areas of soft tissue breakdown due to mechanical loading. Susceptible individuals are subjected to pressure relief strategies to prevent long loading periods. Therefore, ischemia-reperfusion injury may play an important role in the etiology of pressure ulcers. To investigate the inter-relation between postischemic perfusion and changes in skeletal muscle integrity, the hindlimbs of Brown Norway rats were subjected to 4-h ischemia followed by 2-h reperfusion. Dynamic contrast-enhanced MRI was used to examine perfusion, and changes in skeletal muscle integrity were monitored with T-2-weighted MRI. The dynamic contrast-enhanced MRI data showed a heterogeneous postischemic profile in the hindlimb, consisting of areas with increased contrast enhancement (14-76% of the hindlimb) and regions with no-reflow (5-77%). For T-2, a gradual increase in the complete leg was observed during the 4-h ischemic period (from 34 to 41 msec). During the reperfusion phase, a heterogeneous distribution of T-2 was observed. Areas with increased contrast enhancement were associated with a decrease in T-2 (to 38 msec) toward preischemic levels, whereas no-reflow areas exhibited a further increase in T-2 (to 42 msec). These results show that reperlusion after prolonged ischemia may not be complete, thereby continuing the ischemic condition and aggravating tissue damage. Magn Reson Med 66:528-537, 2011. (C) 2011 Wiley-Liss, Inc.