Investigation of germplasm in chrysanthemum cultivars with light-independent coloration.
Investigation of germplasm in chrysanthemum cultivars with light-independent coloration.
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DOI：
10.17660/actahortic.2017.1185.9
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发表时间：
2017-01-01
期刊：
影响因子：
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通讯作者：
Dai, S.
中科院分区：
文献类型：
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作者：
Huang, H.;Li, Y.;Dai, S.
花色是观赏植物最重要的观赏品质。花色苷是决定菊花颜色的主要色素，其生物合成依赖于光照。以372个盆栽菊花品种为试材，在蕾期进行遮光处理，用肉眼观察其花色表型变化。我们有6个品种在遮光处理下可以积累花青苷。通过色素分析发现，B40在遮光处理下花色素苷含量比对照增加近2倍。RT-PCR分析表明，与对照相比，遮荫处理对B40花青素合成途径基因MYB 6、DFR和ANS的转录水平没有影响。然而，这些基因在光依赖型栽培品种B14中不表达。因此，B40细胞中存在一条非光依赖的信号转导途径，能够诱导ABP基因的表达。B40是选育耐光菊花品种的重要品种。本工作也将为深入研究高等植物花色素苷生物合成的分子机制奠定基础。
Flower color is the most important ornamental quality for ornamental plants. Anthocyanin is the main pigment determining the color of chrysanthemums, and its biosynthesis is sunlight-dependent. In this study, we performed shading treatment at the bud stage of 372 potted chrysanthemum cultivars, and we observed the phenotypic change of flower color by the naked eye. We had six cultivars that could accumulate anthocyanin under shading treatment. According to pigment analysis, we found that in B40, the anthocyanin content increased almost two times more under the shading treatment than under control conditions. RT-PCR analysis showed that in B40, the transcriptional levels of anthocyanin biosynthesis pathway genes, including MYB6, DFR and ANS, were not changed under the shading treatment compared with the control conditions. However, these genes were not expressed in the light-dependent cultivar B14. We concluded there is a light independent signal transduction pathway in B40 that can induce the expression of ABP genes. B40 is an important cultivar for breeding light-independent chrysanthemum cultivars. Our work will also lay the foundation for the analysis of the molecular mechanism of anthocyanin biosynthesis in higher plants.