Lens epithelium-derived growth factor (LEDGF) delays photoreceptor degeneration in explants of rd/rd mouse retina
Lens epithelium-derived growth factor (LEDGF) delays photoreceptor degeneration in explants of rd/rd mouse retina
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DOI：
10.1097/00001756-200109170-00039
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发表时间：
2001-09-17
期刊：
影响因子：
1.7
通讯作者：
van Veen, T
中科院分区：
文献类型：
--
作者：
Ahuja, P;Caffé, AR;van Veen, T
晶状体上皮源性生长因子（LEDGF）对血清饥饿和热应激的鸡胚感光细胞、Lewis大鼠光损伤的感光细胞和RCS大鼠的感光细胞均有保护作用。我们的研究目的是通过长期无血清器官培养，研究LEDGF对rd/rd小鼠光感受器细胞的拯救作用。在21-26天的培养期结束时，与未处理的对照组相比，LEDGF处理的小鼠视网膜的光感受器存活率增加。当RPE存在时，LEDGF对视蛋白和阻滞蛋白在视杆感光细胞中的表达和定位没有影响。LEDGF对视网膜感光细胞的保护作用与BDNF相似。已知LEDGF可激活热休克蛋白（Hsps），据报道，升高的热休克蛋白也可抑制细胞凋亡。(C) 2001 Lippincott Williams & Wilkins。
Lens epithelium derived growth factor (LEDGF) has been shown to rescue embryonic chick photoreceptor cells from serum starvation and heat stress, light damaged photoreceptor cells in Lewis rats, and photoreceptor cells in RCS rats. The aim of our study is to study the rescue effect of LEDGF on photoreceptor cells in the rd/rd mouse using our long-term serum free organ culture. At the end of this culture period of 21-26 days LEDGF treated rd mouse retina showed an increased photoreceptor survival compared to the untreated controls. LEDGF has no effect on expression and localization of opsin and arrestin in the rod photoreceptor cells when RPE is present. The protective potency of LEDGF on the retinal photoreceptor cells is similar to that of BDNF. LEDGF is known to activate heat shock proteins (Hsps) and the elevated Hsps are also reported to suppress apoptosis. NeuroReport 12:2951-2955 (C) 2001 Lippincott Williams & Wilkins.