3D printed nervous system on a chip.
3D printed nervous system on a chip.
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DOI：
10.1039/c5lc01270h
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发表时间：
2016-04-21
期刊：
影响因子：
6.1
通讯作者：
McAlpine MC
中科院分区：
文献类型：
--
作者：
Johnson BN;Lancaster KZ;Hogue IB;Meng F;Kong YL;Enquist LW;McAlpine MC
生物启发的器官水平体外平台正在成为基础研究，药物发现和个性化医疗保健的有效技术。特别是，由于神经现象的复杂性以及与开发神经疾病的靶向治疗相关的挑战，神经系统研究的模型尤为重要。在这里，我们介绍了一种基于增材制造的方法，其形式是生物启发的，可定制的3D打印神经系统芯片（3DNSC），用于研究神经系统中的病毒感染。微挤出3D打印策略能够组装仿生支架组件（微通道和隔室），用于轴突网络的对齐和细胞组件的空间组织。使用多尺度仿生装置的神经系统感染的生理学相关研究证明了体外平台的功能。我们发现，雪旺细胞参与轴突到细胞的病毒传播，但似乎难以感染，表现出感染复数（MOI）的1.4个基因组每个细胞。这些结果表明，3D打印是芯片技术上定制模型神经系统原型的一种有价值的方法。
Bioinspired organ-level in vitro platforms are emerging as effective technologies for fundamental research, drug discovery, and personalized healthcare. In particular, models for nervous system research are especially important, due to the complexity of neurological phenomena and challenges associated with developing targeted treatment of neurological disorders. Here we introduce an additive manufacturing-based approach in the form of a bioinspired, customizable 3D printed nervous system on a chip (3DNSC) for the study of viral infection in the nervous system. Micro-extrusion 3D printing strategies enabled the assembly of biomimetic scaffold components (microchannels and compartmented chambers) for the alignment of axonal networks and spatial organization of cellular components. Physiologically relevant studies of nervous system infection using the multiscale biomimetic device demonstrated the functionality of the in vitro platform. We found that Schwann cells participate in axon-to-cell viral spread but appear refractory to infection, exhibiting a multiplicity of infection (MOI) of 1.4 genomes per cell. These results suggest that 3D printing is a valuable approach for the prototyping of a customized model nervous system on a chip technology.